High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus)

Molecular Biology Reports
Zhenbing WuAihua Li

Abstract

Increasing attention has been attracted to host microbiota, due to their vital impact on host health. Little is known about the microbiota of the Chinese giant salamander (Andrias davidianus), in spite of the high economic and scientific value of this endangered species. This study was designed to characterise and compare the gut and lung prokaryotic communities of the Chinese giant salamander by high-throughput sequencing. Our study showed that the giant salamander had a lung prokaryotic community that clustered separately from its intestinal microbiota. Statistical analysis (LEfSe) revealed that the bacterial populations were dominated by Geobacter, Sulfurimonas, and Dechloromonas from Proteobacteria phylum, and Corynebacterium from Actinobacteria phylum in the lung, while Parabacteroides, Bacteroides, and PW3 from Bacteroidetes phylum, and Oscillospira from Firmicutes phylum were predominant in the intestine. A particularly innovative finding was the fairly high abundance of Archaea, especially methanogenic Euryarchaeota. The gut dominant Archaea were Methanocorpusculum and Thermoplasmata vadinCA11, while Methanosaeta and Methanoculleus were the main Archaea in the lung. PICRUSt analysis revealed differentiated functional pr...Continue Reading

References

Jul 31, 1999·Science·G Vogel
Mar 28, 2003·Nature·Ke-Qin Gao, Neil H Shubin
Apr 7, 2004·Applied and Environmental Microbiology·Le H DucSimon M Cutting
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Dec 22, 2006·Nature·Peter J TurnbaughJeffrey I Gordon
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Sep 17, 2008·Nature Reviews. Microbiology·Ruth E LeyJeffrey I Gordon
Jan 7, 2010·PloS One·Markus HiltyWilliam O C Cookson
Jun 4, 2010·The ISME Journal·Elizabeth K CostelloRob Knight
Jan 22, 2011·Archaea : an International Microbiological Journal·H-P Horz, G Conrads
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Dec 17, 2011·Emerging Infectious Diseases·Wuzi DongWenxian Zeng
Jun 12, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·James M BeckGary B Huffnagle
Oct 20, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Tung Nguyen-HieuMichel Drancourt
Jan 18, 2013·Archaea : an International Microbiological Journal·Christoph WredeMichael Hoppert
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower
Nov 20, 2013·Environmental Microbiology Reports·Kevin D KohlM Denise Dearing
Nov 22, 2013·FEMS Microbiology Ecology·Jiajia NiTanglin Zhang
Jan 1, 2014·BMC Microbiology·Kenneth Klingenberg BarfodKaren Angeliki Krogfelt
Jan 7, 2014·Nature Medicine·Aurélien TrompetteBenjamin J Marsland
Feb 6, 2014·PloS One·Pascale Blais LecoursCaroline Duchaine
Apr 1, 2014·Cell·Yasmine Belkaid, Timothy W Hand
Jul 26, 2014·Bioinformatics·Donovan H ParksRobert G Beiko
Mar 12, 2016·BMC Microbiology·Chun-Wen ChangPei-Chun Liao
Sep 30, 2016·Microbial Ecology·Obed Hernández-GómezRod N Williams
Apr 6, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michel DrancourtDidier Raoult
Jun 24, 2017·Microbiome·Nisha SinghAshwani Kumar
Oct 28, 2017·Journal of Oncology·Rea BingulaEdith Filaire
Dec 6, 2017·Molecular Ecology Resources·Elin VidevallCharlie K Cornwallis
Mar 27, 2018·Seminars in Respiratory and Critical Care Medicine·Alicia B Mitchell, Allan R Glanville
Jul 12, 2018·Scientific Reports·Mohammad Jaber AlipourMikael Niku
Mar 25, 2019·Nature Communications·Yun ZhaoHans-Christian Reinecker

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
DRA005870

Methods Mentioned

BETA
dissection
electrophoresis
PCR

Software Mentioned

QIIME Pipeline
Phylogenetic Investigation of Communities by Reconstruction of...
PERMANOVA
LEfSe
PICRUSt
SPSS
Statistical Analysis of Metagenomics Profiles ( STAMP )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.