High throughput two-dimensional blue-native electrophoresis: a tool for functional proteomics of cytoplasmatic protein complexes from Chlorobium tepidum.

Photosynthesis Research
Michalis AivaliotisG Tsiotis

Abstract

Chl. tepidum is a Gram-negative green-sulfur bacterium, which is strict by anaerobic and grows by utilizing sulfide or thiosulfate as an electron source. Blue native-polyacrylamide gel electrophoresis (BN-PAGE) is widely used for the analysis of oligomeric state and molecular mass non-dissociated protein complexes. In this study, a number of proteomic techniques were used to investigate the oligomeric state enzymes. In particular, the Chl. tepidum-soluble proteome was monitored under native condition by using BN-PAGE. The BN-PAGE protein complexes map was analyzed by MALDI-TOF MS after trypsin treatment and from 42 BN proteins bands, 62 different proteins were identified. Additionally, functional information regarding protein-protein interactions was assembled, by coupling 2-D BN-PAGE with MALDI-TOF MS. One-hundred and seventy gel bands were spotted, out of which 187 different proteins were identified. The identified proteins belong to various functional categories like energy metabolism, protein synthesis, amino acid biosynthesis, central intermediate metabolism, and biosynthesis of cofactors indicating the potential of the method for elucidation of functional proteomes.

References

Aug 18, 1999·Electrophoresis·H ThiellementM Zivy
May 3, 2001·Electrophoresis·W M LauberJ P Malone
Jun 8, 2001·Annual Review of Biochemistry·M MannA Pandey
Nov 1, 2001·Biochimica Et Biophysica Acta·G HauskaG Tsiotis
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Leo G J NijtmansIan J Holt
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jonathan A EisenClaire M Fraser
Jun 24, 2004·Biochimica Et Biophysica Acta·Irene TsirogianniGeorgios Tsiotis
Feb 15, 2005·Plant Physiology and Biochemistry : PPB·Geneviève EphritikhineNorbert Rolland
Oct 18, 2005·Photosynthesis Research·Hervé-W RémigyGeorgios Tsiotis
Oct 26, 2005·Photosynthesis Research·Norbert RollandJacques Joyard
Oct 26, 2005·Photosynthesis Research·Julian P Whitelegge

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Citations

Sep 2, 2011·The Journal of Biological Chemistry·Matthew D DunBrett Nixon
Aug 12, 2014·Journal of Proteomics·Michelle D GlewEric C Reynolds
Sep 4, 2008·Proteomics·Ilka Wittig, Hermann Schägger
Feb 23, 2010·Microbiological Research·Jiri DreslerJiri Stulik
Jun 19, 2007·Biochimica Et Biophysica Acta·Konstantinos KantzilakisGeorgios Tsiotis
Oct 13, 2006·Proteomics·Veronika Reisinger, Lutz Andreas Eichacker
Jun 6, 2018·Journal of Molecular Modeling·Xia-Min HuZhen-Li Min

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