High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces

Micromachines
Batzorig LombodorjFan-Gang Tseng

Abstract

A microfluidic chip, which can separate and enrich leukocytes from whole blood, is proposed. The chip has 10 switchback curve channels, which are connected by straight channels. The straight channels are designed to permit the inertial migration effect and to concentrate the blood cells, while the curve channels allow the Dean flow to further classify the blood cells based on the cell sizes. Hydrodynamic suction is also utilized to remove smaller blood cells (e.g., red blood cell (RBC)) in the curve channels for higher separation purity. By employing the inertial migration, Dean flow force, and hydrodynamic suction in a continuous flow system, our chip successfully separates large white blood cells (WBCs) from the whole blood with the processing rates as high as 1 × 108 cells/sec at a high recovery rate at 93.2% and very few RBCs (~0.1%).

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Citations

Sep 11, 2020·Lab on a Chip·Wenlai TangNan Xiang
Apr 21, 2021·Scientific Reports·Tharagan KumarAman Russom
Sep 13, 2021·Electrophoresis·Sharath Narayana IyengarAman Russom
Nov 12, 2021·The Analyst·Xuefeng XuMing Yin

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Methods Mentioned

BETA
size-based filtration
chip
confocal microscopy
hydrophoretic filtration

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