Abstract
A microfluidic chip, which can separate and enrich leukocytes from whole blood, is proposed. The chip has 10 switchback curve channels, which are connected by straight channels. The straight channels are designed to permit the inertial migration effect and to concentrate the blood cells, while the curve channels allow the Dean flow to further classify the blood cells based on the cell sizes. Hydrodynamic suction is also utilized to remove smaller blood cells (e.g., red blood cell (RBC)) in the curve channels for higher separation purity. By employing the inertial migration, Dean flow force, and hydrodynamic suction in a continuous flow system, our chip successfully separates large white blood cells (WBCs) from the whole blood with the processing rates as high as 1 × 108 cells/sec at a high recovery rate at 93.2% and very few RBCs (~0.1%).
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Sep 11, 2020·Lab on a Chip·Wenlai TangNan Xiang
Apr 21, 2021·Scientific Reports·Tharagan KumarAman Russom
May 20, 2021·Electrophoresis·Xiaoguang LuYe Ai
Sep 13, 2021·Electrophoresis·Sharath Narayana IyengarAman Russom
Nov 12, 2021·The Analyst·Xuefeng XuMing Yin