High-throughput wide dynamic range procedure for the simultaneous quantification of nicotine and cotinine in multiple biological matrices using hydrophilic interaction liquid chromatography-tandem mass spectrometry

Analytical and Bioanalytical Chemistry
Raúl Pérez-OrtuñoJosé A Pascual

Abstract

A straightforward, high-throughput method was developed and fully validated for the simultaneous determination of the specific tobacco biomarkers nicotine and its main metabolite cotinine in a wide dynamic range and supporting the most common human biological matrices (urine, oral fluid and hair). Sample preparation was performed inside the very HPLC injection vials by pipetting 0.5 mL of the liquid samples, deuterated internal standards in alkaline solution and dichloromethane as extraction solvent. Solid samples (i.e. around 10 mg hair) were first submitted to alkaline digestion in the HPLC vials and processed accordingly. The organic phase (reached through the upper aqueous layer) was directly injected without further treatment. Instrumental analysis was performed using hydrophilic interaction (HILIC) ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Total chromatographic time was 2 min. The method covers a wide dynamic range making it fit-for-purpose for the analysis of samples covering entire populations, irrespective of the level of exposure or tobacco use. Calibration curves (r (2) > 0.995) covered the range 1-2000 ng/mL (or 0.05-100 ng/mg hair) for nicotine and 0.1-2000 ng/mL (or 0.005...Continue Reading

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Citations

Mar 22, 2018·Bioanalysis·Marta Concheiro, Marilyn A Huestis
Apr 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Junhee KimSang Beom Han
Mar 7, 2021·International Journal of Environmental Research and Public Health·Hernâni MarquesEugenia Gallardo
Nov 2, 2020·Environmental Research·Cristina Lidón-MoyanoJose M Martínez-Sánchez

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