Abstract
Even Non-alcoholic steatohepatitis (NASH) has been becoming the key role in process of liver fibrosis or cirrhosis, no any NASH involving liver fibrosis mice model which consistent with the mechanisms of fatty acid and glucose metabolism disorder was widely accepted. Here, we established a mouse model of nonalcoholic steatohepatitis (NASH) with liver fibrosis using a high-fat, high-carbohydrate diet (HFHC) and analyzed the potential pathogenesis using a transcriptome microarray. Fifty mice were stratified by weight and randomly divided into the HFHC model and control (Con) groups. Ten mice were sacrificed at the beginning of the experiments, 10 mice of HFHC and Con group were euthanized at the end of 20 and 30 weeks. The following analyses were performed: biochemical analysis; histological assessment; evaluation of hepatic type I collagen (Col-I), α-smooth muscle actin (α-SMA) and transforming growth factor-β1 (TGF-β1) protein and mRNA expression levels; and transcriptomic gene chip analysis. Compared with the Con group at each time point, the body weight and liver wet weight of the HFHC model group of mice were significantly higher. At 30th weeks, alanine aminotransferase (ALT), aspartate aminotransferase (AST), fasting blood ...Continue Reading
References
Oct 22, 2003·Journal of Hepatology·Jacob GeorgeGeoffrey Farrell
Jan 27, 2006·International Journal of Experimental Pathology·Quentin M Anstee, Robert D Goldin
Feb 27, 2007·Biochimica Et Biophysica Acta·Yumi KondohReiko Urade
Jun 5, 2007·Clinics in Liver Disease·Roslyn M London, Jacob George
Sep 6, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laura H TetriBrent A Neuschwander-Tetri
Nov 13, 2008·Chemico-biological Interactions·O LeeP J O'Brien
Apr 2, 2010·The Journal of Nutrition·Roberta M MachadoAna Maria P Lottenberg
May 14, 2010·Journal of Hepatology·Noriyuki ObaraTooru Shimosegawa
Jul 8, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rohit KohliRandy J Seeley
Apr 25, 2013·Clinical and Experimental Pharmacology & Physiology·Norihiro KubotaFumiyasu Ishikawa
Aug 16, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mattias EkstedtRolf Hultcrantz
May 4, 2015·Gastroenterology·Paul AnguloFlemming Bendtsen
Nov 7, 2016·The Journal of Nutritional Biochemistry·Xiao HuToshifumi Aoyama
Jul 6, 2017·European Journal of Nutrition·Sugeedha JeyapalAhamed Ibrahim
Aug 15, 2017·Journal of Hepatology·Hannes HagströmStergios Kechagias
Nov 23, 2017·The New England Journal of Medicine·Anna M Diehl, Christopher Day
Citations
Apr 1, 2021·Life Sciences·Jian ZhouEn-Sheng Ji
May 22, 2021·Frontiers in Pharmacology·Cheng ChenQin Feng
Jun 8, 2021·BioMed Research International·Xin XinQin Feng
Dec 10, 2021·Frontiers in Nutrition·Xin XinFeng Qin