High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in mucosa of HIV-1-exposed seronegative individuals

PloS One
Wbeimar Aguilar-JiménezMaría Teresa Rugeles

Abstract

Vitamin D (VitD) is an endogenous immunomodulator that could protect from HIV-1 infection reducing immune activation and inducing the expression of anti-HIV-1 peptides. To establish a correlation between VitD and natural resistance to HIV-1 infection, a case-control study using blood and mucosa samples of 58 HIV-1-exposed but seronegative (HESN) individuals, 43 HIV-1 seropositives (SPs) and 59 non-exposed healthy controls (HCs) was carried out. The VitD concentration in plasma was determined by ELISA, and mRNA relative units (RU) of VDR, IL-10, TGF-β, TNF-α and IL-1β in peripheral blood mononuclear cells (PBMCs), oral and genital mucosa was quantified by qRT-PCR. mRNA levels of human beta-defensin (HBD) -2 and -3 were previously reported and used for correlations. Significantly higher levels of VitD were found in plasma as well as higher mRNA RU of VDR in PBMCs, and in genital mucosa from HESN compared to HCs. In addition, higher mRNA RU of TNF-α, IL-1β and IL-10, and lower mRNA RU of TGF-β were found in PBMC from HESNs compared to HCs. We also observed higher IL-10 mRNA RU in genital mucosa of HESNs compared to HCs, and the mRNA levels of TNF-α in oral and genital mucosa of SPs were higher compared to HESNs. Furthermore, posit...Continue Reading

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Citations

Apr 17, 2015·Poultry Science·Hyun-Jun JangMi Na Park
Jun 14, 2016·Advances in Virology·John F Arboleda, Silvio Urcuqui-Inchima
Jan 8, 2019·Reviews in Medical Virology·Majid Teymoori-RadSayed Mahdi Marashi
Feb 23, 2020·Expert Review of Clinical Immunology·Claudio FeniziaMara Biasin
Aug 11, 2016·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Wbeimar Aguilar-JimenezMaria T Rugeles
Feb 22, 2017·Hormone Molecular Biology and Clinical Investigation·Simon Villegas-OspinaMaría T Rugeles
Mar 30, 2018·Frontiers in Immunology·María Ángeles Jiménez-SousaSalvador Resino
Jul 2, 2019·Genetics and Molecular Biology·Ronaldo Celerino da SilvaJaqueline de Azevêdo Silva
Jul 9, 2021·Frontiers in Molecular Biosciences·Santosh K Ghosh, Aaron Weinberg

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Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

GraphPad Prism

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