High transposition rates of Osvaldo, a new Drosophila buzzatii retrotransposon

Molecular & General Genetics : MGG
M Labrador, A Fontdevila

Abstract

Transposition of a new Drosophila retrotransposon was investigated. Total genomic Southern analysis and polytene in situ hybridizations in D. buzzatii strains and other related species using a 6 kb D. buzzatii clone (cDb314) showed a dispersed, repetitive DNA pattern, suggesting that this clone contains a transposable element (TE). We have sequenced the cDb314 clone and demonstrated that it contains all the conserved protein sequences and motifs typical of retrovirus-related sequences. Although cDb314 does not include the complete TE, the protein sequence alignment demonstrates that it includes a defective copy of a new long terminal repeat (LTR) retrotransposon, related to the gypsy family, which we have named Osvaldo. Using a D. buzzatii inbred line in which all insertion sites are known, we have measured Osvaldo transposition rates in hybrids between this D. buzzatii line and its sibling species D. koepferae. The results show that Osvaldo transposes in bursts at high rate, both in the D. buzzatii inbred line and in species hybrids.

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Citations

Aug 30, 1995·Molecular & General Genetics : MGG·I Marín, A Fontdevila
Oct 14, 2006·Molecular Genetics and Genomics : MGG·M P García Guerreiro, A Fontdevila
May 28, 2011·Molecular Genetics and Genomics : MGG·Adriana GranzottoClaudia M A Carareto
Oct 6, 2011·Heredity·M P García Guerreiro
Jun 26, 2002·Molecular Biology and Evolution·Cristina VieiraChristian Biémont
Aug 12, 2005·Cytogenetic and Genome Research·A Fontdevila
Apr 26, 2011·BMC Evolutionary Biology·María Pilar García Guerreiro, Antonio Fontdevila
Mar 4, 2014·PloS One·Doris VelaMaría Pilar García Guerreiro
Sep 13, 2015·Insect Biochemistry and Molecular Biology·R D Fernández-MedinaC M A Carareto
Apr 17, 2003·FEMS Microbiology Reviews·J Kalervo HiltunenAner Gurvitz
Aug 15, 2018·The Journal of Heredity·Antonio Fontdevila

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