High voltage-activated Ca(2+) channel currents in isolectin B(4)-positive and -negative small dorsal root ganglion neurons of rats

Neuroscience Letters
Zi-Zhen Wu, Hui-Lin Pan

Abstract

Voltage-gated Ca(2+) channels in the primary sensory neurons are important for neurotransmitter release and regulation of nociceptive transmission. Although multiple classes of Ca(2+) channels are expressed in the dorsal root ganglion (DRG) neurons, little is known about the difference in the specific channel subtypes among the different types of DRG neurons. In this study, we determined the possible difference in high voltage-activated Ca(2+) channel currents between isolectin B(4) (IB(4))-positive and IB(4)-negative small-sized (15-30 microm) DRG neurons. Rat DRG neurons were acutely isolated and labeled with IB(4) conjugated to a fluorescent dye. Whole-cell patch clamp recordings of barium currents flowing through calcium channels were performed on neurons with and without IB(4). The peak current density of voltage-gated Ca(2+) currents was not significantly different between IB(4)-positive and IB(4)-negative neurons. Also, both nimodipine and omega-agatoxin IVA produced similar inhibitory effects on Ca(2+) currents in these two types of neurons. However, block of N-type Ca(2+) channels with omega-conotoxin GVIA produced a significantly greater reduction of Ca(2+) currents in IB(4)-positive than IB(4)-negative neurons. Furth...Continue Reading

References

May 15, 1998·Neuron·W D Snider, S B McMahon
Nov 21, 1998·The European Journal of Neuroscience·L VulchanovaR Elde
Dec 12, 2001·Neuroscience·L VulchanovaC N Honda

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Citations

Nov 19, 2009·American Journal of Physiology. Endocrinology and Metabolism·Yi Wang, Xinfu Guan
Sep 8, 2010·Neuroscience Letters·Kentaro OnoKiyotoshi Inenaga
Jun 6, 2009·The American Journal of Gastroenterology·Ai-Hua QianYao-Zong Yuan
May 11, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Zi-Zhen Wu, Hui-Lin Pan
Aug 30, 2005·The Journal of Pharmacology and Experimental Therapeutics·Pál KocsisIstván Tarnawa
Oct 29, 2015·The Journal of Biological Chemistry·Meng-Hua ZhouHui-Lin Pan

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