PMID: 6971875Sep 1, 1980Paper

High-voltage electron microscopy of crossbridge interactions in striated muscle

Journal of Muscle Research and Cell Motility
A FreundlichJ M Squire

Abstract

In order to investigate the geometry of the interactions which myosin molecules make with actin filaments we have studied thick (0.2--0.5 micrometer) transverse sections of striated muscles in the 1 Me V electron microscope at Imperial College. Sections obtained from fixed relaxed frog sartorius muscle and both fixed relaxed and fixed rigor insect flight muscles, show regular electron opaque features between the thick and thin filament profiles. These are thought to be the overlapping images of the many levels of myosin heads that occur in such sections. From the appearances of these images, together with studies of thin transverse sections, it appears that of the possible interactions which one myosin molecule can make, namely that its two component heads interact with the same thin filament or with two different thin filaments, it is the former interaction (both heads on the same filament) which is predominant. Nevertheless appearances have been seen similar to those expected if an interaction of one molecule with two thin filaments occurs. It is concluded that both single filament and two filament interactions can occur depending on the steric convenience of the available actin subunits, but that the single filament interact...Continue Reading

References

Dec 1, 1977·Journal of Microscopy·M Sjöström, J M Squire
Jan 1, 1978·Progress in Biophysics and Molecular Biology·E Eisenberg, T L Hill
Apr 15, 1978·Journal of Molecular Biology·R Cooke, K E Franks
Oct 9, 1979·Journal of Molecular Biology·J Trinick, G Offer
Jan 26, 1978·Nature·G Offer, A Elliott
Aug 25, 1978·Journal of Molecular Biology·A Elliott, G Offer
Nov 5, 1978·Journal of Molecular Biology·P Luther, J Squire
Jan 1, 1975·Annual Review of Biophysics and Bioengineering·J M Squire
Apr 5, 1976·Journal of Molecular Biology·R Craig, G Offer
Jun 20, 1969·Science·H E Huxley
Jan 1, 1967·Progress in Biophysics and Molecular Biology·J W Pringle
May 28, 1969·Journal of Molecular Biology·S LoweyH Baker
Mar 5, 1973·Journal of Molecular Biology·S S Margossian, S Lowey
Jun 25, 1973·Journal of Molecular Biology·R T Tregear, J M Squire
Oct 22, 1971·Nature·A F Huxley, R M Simmons
Mar 28, 1972·Journal of Molecular Biology·E Rome
Sep 14, 1972·Journal of Molecular Biology·A Miller, R T Tregear
Jun 14, 1970·Journal of Molecular Biology·P B MooreD J DeRosier
Nov 14, 1966·Journal of Molecular Biology·R A Chaplain, R T Tregear
Feb 14, 1980·Nature·J Seymour, E J O'Brien
Aug 25, 1980·Journal of Molecular Biology·P K Luther, J M Squire

❮ Previous
Next ❯

Citations

Aug 1, 1984·Journal of Muscle Research and Cell Motility·E Varriano-MarstonJ C Haselgrove
Dec 1, 1987·Journal of Muscle Research and Cell Motility·M C ReedyR S Goody
Aug 1, 1993·Journal of Muscle Research and Cell Motility·K Hirose, T Wakabayashi
Oct 5, 1981·Journal of Molecular Biology·G OfferA Elliott
Sep 15, 1983·Journal of Molecular Biology·A Freundlich, J M Squire

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.