PMID: 7540052Apr 1, 1995Paper

High water permeability of human spermatozoa is mercury-resistant and not mediated by CHIP28

Biology of Reproduction
C LiuJ K Critser

Abstract

A novel integral membrane protein with an apparent molecular mass of 28 kDa (CHIP28) was first isolated from human erythrocytes and is now recognized as a water channel protein. The expression of this protein has been found in several other cell types that all require high water permeability for their functions. Recent studies have shown that the water permeability (Lp) of human spermatozoa is among the highest reported for mammalian cells. Together with the low activation energy of human spermatozoa for Lp, this suggests that CHIP28 water channel may be present in the plasma membrane of human spermatozoa. However, our current studies do not support this hypothesis. Results from Western blot analysis on human sperm plasma membrane proteins, performed through use of an antibody against human erythrocyte CHIP28 protein, indicated that human spermatozoa do not express CHIP28 protein on their cell surface (n = 10). Consistent with the Western blot finding, mercuric chloride (HgCl2), a known water channel blocker, failed to reduce the osmotic water permeability of human spermatozoa. The calculated Lp values were 1.30 +/- 0.29 micron/min/atm (n = 16; mean +/- SEM) for the control group and 1.31 +/- 0.29 (n = 9; mean +/- SEM), 1.04 +/...Continue Reading

Citations

Apr 28, 2004·Molecular and Cellular Endocrinology·Trevor G CooperPetra Sipilä
Oct 22, 2002·BJOG : an International Journal of Obstetrics and Gynaecology·Christine M Y ChoyChristopher J Haines
Jul 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·C CalliesC H Yeung
Feb 22, 2012·Molecular Aspects of Medicine·Dan ZhangHe-Feng Huang
Oct 18, 2008·Cryobiology·Junya ItoNaomi Kashiwazaki
May 23, 2001·Biology of Reproduction·G CalamitaM Svelto
Oct 27, 2007·International Journal of Toxicology·Hooi-Hoon Ang, Kheng-Leng Lee
Jul 15, 2006·Human Reproduction Update·He-Feng HuangYing-Ying Ma
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·M YesteN Prieto-Martínez
Jan 4, 2017·International Journal of Molecular Sciences·Umberto LaforenzaGiulia Gastaldi

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