High yield of cells committed to the photoreceptor fate from expanded mouse retinal stem cells

Stem Cells
Faten Merhi-SoussiYvan Arsenijevic

Abstract

The purpose of the present work was to generate, from retinal stem cells (RSCs), a large number of cells committed toward the photoreceptor fate in order to provide an unlimited cell source for neurogenesis and transplantation studies. We expanded RSCs (at least 34 passages) sharing characteristics of radial glial cells and primed the cells in vitro with fibroblast growth factor (FGF)-2 for 5 days, after which cells were treated with the B27 supplement to induce cell differentiation and maturation. Upon differentiation, cells expressed cell type-specific markers corresponding to neurons and glia. We show by immunocytochemistry analysis that a subpopulation of differentiated cells was committed to the photoreceptor lineage given that these cells expressed the photoreceptor proteins recoverin, peripherin, and rhodopsin in a same ratio. Furthermore, cells infected during the differentiation procedure with a lentiviral vector expressing green fluorescent protein (GFP) under the control of either the rhodopsin promoter or the interphotoreceptor retinoid-binding protein (IRBP) promoter, expressed GFP. FGF-2 priming increased neuronal differentiation while decreasing glia generation. Reverse transcription-polymerase chain reaction ana...Continue Reading

References

Mar 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P A Hargrave, J H McDowell
Jan 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K H Jones, J A Senft
Apr 28, 1988·Nature·T Watanabe, M C Raff
Jan 1, 1993·Visual Neuroscience·A H MilamA M Dizhoor
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·C L CepkoD Ezzeddine
Nov 1, 1995·The Journal of General Physiology·Y KoutalosK W Yau
Jun 3, 1996·Brain Research·S Zhao, C J Barnstable
Aug 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K TomitaR Kageyama
Dec 2, 1999·Nature Genetics·T FurukawaC L Cepko
Feb 9, 2000·Molecular and Cellular Neurosciences·A A DavisT A Reh
Feb 19, 2000·Biochemical and Biophysical Research Communications·D M ChackoI Ahmad
Mar 17, 2000·Science·V TropepeD van der Kooy
Feb 7, 2001·Biochemical and Biophysical Research Communications·I AhmadH Pham
Jan 3, 2001·Developmental Biology·E HartfussM Götz
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·W KedzierskiG H Travis
Nov 13, 2001·Nature Neuroscience·M HarutaM Takahashi
Apr 17, 2003·Neuroscience Letters·Tadamichi AkagiMasayo Takahashi
Jul 25, 2003·Developments in Ophthalmology·M M HimsC F Inglehearn
Aug 21, 2003·The British Journal of Ophthalmology·E J MayerA D Dick
Nov 13, 2003·Vision Research·Federico Gonzalez-Fernandez
Mar 24, 2004·Seminars in Cell & Developmental Biology·Jun Hatakeyama, Ryoichiro Kageyama
Mar 30, 2004·Neuron·Tracy L Young, Constance L Cepko
Jul 13, 2004·Experimental Neurology·Maeve A CaldwellClive N Svendsen
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Brenda L K ColesDerek van der Kooy
Nov 24, 2004·Investigative Ophthalmology & Visual Science·Tadamichi AkagiMasayo Takahashi
Mar 16, 2005·American Journal of Ophthalmology·Stuart L Fine

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Citations

Oct 4, 2007·Cell and Tissue Research·Meta W Djojosubroto, Yvan Arsenijevic
Feb 23, 2012·Cell and Tissue Research·Christian W SchmeerStefan Isenmann
May 9, 2009·Current Opinion in Ophthalmology·Paul S Baker, Gary C Brown
Oct 3, 2012·PloS One·Dominic EberleMarius Ader
Feb 15, 2011·Pakistan Journal of Biological Sciences : PJBS·M Safari
Jul 20, 2010·Experimental Eye Research·Fiona C ManserghG Jane Farrar
Jul 22, 2010·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Brian G Ballios, Derek van der Kooy
Oct 26, 2007·Seminars in Cell & Developmental Biology·Graziella PellegriniYvan Arsenijevic
Dec 3, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Henry K Yip
Jan 22, 2014·Journal of Cellular and Molecular Medicine·Isotta ChimentiAlessandro Giacomello
Feb 23, 2012·Progress in Retinal and Eye Research·Stefanie G WohlStefan Isenmann
Jun 18, 2014·Stem Cell Reports·Sarah DecembriniYvan Arsenijevic
Jan 24, 2017·Progress in Retinal and Eye Research·Melissa K JonesShaomei Wang
Jan 21, 2017·Frontiers in Systems Neuroscience·Tiago F Santos-FerreiraMarius Ader
Aug 30, 2017·International Journal of Molecular Medicine·Ying LiuJian Ge

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