High yield of parasites and prolonged in vitro culture of Histomonas meleagridis

Avian Pathology : Journal of the W.V.P.A
Harold M J F van der HeijdenWil J M Landman

Abstract

Dwyer's medium is a frequently employed culture medium for Histomonas meleagridis with rice powder as an essential ingredient. The effect of adding larger quantities of rice powder to the culture medium and the influence of the size of the rice particles on the growth of H. meleagridis was examined. Increasing the amount of rice powder from the standard amount of 10 to 12 mg to 50 to 100 mg per 12.5 ml medium resulted in approximately a 10-fold increase of parasites. Larger quantities of rice powder did not give better yields. The particle size of the rice powder proved relatively unimportant, although the addition of only large rice powder particles (>250 microm) resulted in a somewhat lesser yield. H. meleagridis cultures could be prolonged from approximately 4 days to at least 2 weeks without subculturing by supplementing the culture medium with rice powder only.

References

Dec 1, 1970·Experimental Parasitology·M D Ruff, M F Hansen
May 1, 1967·The Journal of protozoology·E E LundA M Chute
Jan 9, 2004·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Katsuhiko YonemuraAkira Hishida
May 1, 1952·Annals of Tropical Medicine and Parasitology·G BERKS, R A NEAL

❮ Previous
Next ❯

Citations

Dec 17, 2008·Parasitology Research·Mareike MunschH M Hafez
Feb 18, 2009·Parasitology Research·Lotfi AbdulRahman, Hafez Mohamed Hafez
Jul 21, 2006·Avian Pathology : Journal of the W.V.P.A·Harold M J F van der HeijdenRon Peek
Feb 7, 2008·Avian Diseases·Harold M J F van der Heijdena, Wil J M Landman
Apr 9, 2008·Veterinary Parasitology·Harold M J F van der Heijden, Wil J M Landman
Jan 13, 2015·Veterinary Parasitology·Michael HessPetra Ganas
Mar 8, 2016·Avian Pathology : Journal of the W.V.P.A·W J M LandmanE Viscogliosi
Jan 19, 2008·Avian Pathology : Journal of the W.V.P.A·Harold M J F van der Heijden, Wil J M Landman

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.