High-Yielding Terpene-Based Biofuel Production in Rhodobacter capsulatus.

ACS Synthetic Biology
Yang ZhangJifeng Yuan

Abstract

Energy crisis and global climate change have driven an increased effort toward biofuel synthesis from renewable feedstocks. Herein, purple nonsulfur photosynthetic bacterium (PNSB) of Rhodobacter capsulatus was explored as a platform for high-titer production of a terpene-based advanced biofuel-bisabolene. A multilevel engineering strategy such as promoter screening, improving the NADPH availability, strengthening the precursor supply, suppressing the side pathways, and introducing a heterologous mevalonate pathway, was used to improve the bisabolene titer in R. capsulatus. The above strategies enabled a 35-fold higher titer of bisabolene than that of the starting strain, reaching 1089.7 mg/L from glucose in a shake flask. The engineered strain produced 9.8 g/L bisabolene with a yield of >0.196 g/g-glucose under the two-phase fed-batch fermentation, which corresponds to >78% of theoretical maximum. Taken together, our work represents one of the pioneering studies to demonstrate PNSB as a promising platform for terpene-based advanced biofuel production.

References

Feb 25, 1988·Nucleic Acids Research·P W PiperS M Kingsman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 5, 2003·Nature Biotechnology·Vincent J J MartinJay D Keasling
Jun 1, 1960·Journal of General Microbiology·W R SISTROM
May 19, 2009·Current Opinion in Microbiology·Mathew A Rude, Andreas Schirmer
Jul 14, 2010·Biochemistry. Biokhimii︠a︡·Zongli HuGuoping Chen
Jul 31, 2010·Science·Andreas SchirmerStephen B del Cardayre
Sep 29, 2011·Nature Communications·Pamela P Peralta-YahyaTaek Soon Lee
Oct 1, 2013·Nature·Yong Jun Choi, Sang Yup Lee
Aug 26, 2014·Frontiers in Bioengineering and Biotechnology·Fiona K DaviesMatthew C Posewitz
Oct 23, 2016·Journal of Biotechnology·Jifeng YuanChi Bun Ching
Nov 9, 2016·Journal of Industrial Microbiology & Biotechnology·Jifeng YuanChi Bun Ching
Nov 17, 2016·Applied Microbiology and Biotechnology·Jifeng YuanChi-Bun Ching
May 29, 2018·Journal of Agricultural and Food Chemistry·Anping SuYonghong Meng
Nov 27, 2019·Microbial Cell Factories·Ioannis MougiakosJohn van der Oost
Oct 11, 2020·Biotechnology and Bioengineering·Enrico OrsiRuud A Weusthuis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.