High yields of stable and highly pure nucleocapsid proteins of different hantaviruses can be generated in the yeast Saccharomyces cerevisiae

Journal of Biotechnology
Ausra RazanskieneRainer G Ulrich

Abstract

Recently, the high-level expression of authentic and hexahistidine (His)-tagged Puumala (strain Vranica/Hällnäs) hantavirus nucleocapsid protein derivatives in the yeast Saccharomyces cerevisiae has been reported [Dargeviciute et al., Vaccine, 20 (2002) 3523-3531]. Here we describe the expression of His-tagged nucleocapsid proteins of other Puumala virus strains (Sotkamo, Kazan) as well as Dobrava (strains Slovenia and Slovakia) and Hantaan (strain Fojnica) hantaviruses using the same system. All nucleocapsid proteins were expressed in the yeast S. cerevisiae at high levels. The nucleocapsid proteins can be easily purified by nickel chelate chromatography; the yield for all nucleocapsid proteins ranged from 0.5 to 1.5 mg per g wet weight of yeast cells. In general, long-term storage of all nucleocapsid proteins without degradation can be obtained by storage in PBS at -20 degrees C or lyophilization. The nucleocapsid protein of Puumala virus (strain Vranica/Hällnäs) was demonstrated to contain only traces of less than 10 pg nucleic acid contamination per 100 microg of protein. The yeast-expressed nucleocapsid proteins of Hantaan, Puumala and Dobrava viruses described here represent useful tools for serological hantavirus diagnos...Continue Reading

References

Mar 1, 1978·The Journal of Infectious Diseases·H W LeeK M Johnson
Sep 3, 1987·Nature·S E AdamsA J Kingsman
Nov 1, 1982·The Journal of Infectious Diseases·H W LeeK M Johnson
Jan 12, 1984·Nature·W J McAleerM R Hilleman
May 11, 1983·Nucleic Acids Research·R A HitzemanH Oppermann
Jan 1, 1995·Archives of Virology·T DzagurovaA Lundkvist
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J E Tavis, D Ganem
Jan 1, 1993·Archives of Virology·S R KlepferE V Jones
Nov 26, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J MustonenA Vaheri
Mar 3, 1999·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H MeiselD H Krüger
Feb 13, 2001·Journal of Virology·A AlfadhliE Barklis
May 29, 2001·Journal of Clinical Microbiology·A PapaO Vapalahti
Jul 18, 2001·The Journal of General Virology·P KaukinenA Plyusnin
Mar 9, 2002·Journal of Virology·Xiaolin XuColleen B Jonsson
Apr 16, 2002·Viral Immunology·A LundkvistR Ulrich
May 23, 2002·The Journal of Biological Chemistry·Ayna AlfadhliEric Barklis
Oct 12, 2002·Journal of Medical Virology·Irina GolovljovaAke Lundkvist
Feb 27, 2003·Intervirology·Rainer UlrichDetlev H Krüger
May 29, 2003·The Journal of General Virology·Annika StoltJoakim Dillner
Sep 6, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Dhanraj SamuelDavid Brown
Mar 9, 2004·Journal of Clinical Microbiology·Boris KlempaDetlev H Krüger

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Citations

Jul 26, 2012·Archives of Virology·Mathias SchlegelBernd Köllner
Jan 12, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H-R XiongZ-Q Yang
Jul 10, 2007·Virus Genes·Indre KucinskaiteRainer G Ulrich
May 25, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jonas SchmidtRainer Ulrich
Nov 13, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sara ChandyGopalan Sridharan
Apr 9, 2010·Clinical Microbiology Reviews·Colleen B JonssonOlli Vapalahti
Jun 16, 2005·Journal of Clinical Microbiology·Boris KlempaDetlev H Krüger
Jun 4, 2014·The Journal of Experimental Medicine·Martin J RafteryGünther Schönrich
Jun 30, 2009·Virus Research·Rasa PetraityteWojtek P Michalski
Jul 3, 2007·Journal of Biotechnology·Mindaugas JuozapaitisWojtek P Michalski
Nov 25, 2006·Virus Research·Mindaugas JuozapaitisWojtek P Michalski
Mar 1, 2006·Journal of Virological Methods·Andreas RangDetlev H Krüger
Sep 16, 2015·BMC Veterinary Research·Justas LazutkaRasa Petraityte-Burneikiene
Nov 4, 2016·Epidemiology and Infection·D ReilJ Jacob
Sep 6, 2013·Clinical and Vaccine Immunology : CVI·Dace SkrastinaPaul Pumpens
Dec 20, 2008·Expert Review of Vaccines·Piet MaesMarc Van Ranst
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wiebke HandkeAndreas Rang
Jul 1, 2020·Frontiers in Cellular and Infection Microbiology·Martin J RafteryGünther Schönrich
Feb 23, 2020·Frontiers in Microbiology·Rongrong LiuXingan Wu
Sep 6, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Stephan DrewesRainer G Ulrich
Oct 16, 2020·Virus Research·Aliona ŠpakovaRasa Petraitytė-Burneikienė
Nov 27, 2020·Virus Research·Justas LazutkaPaulius Lukas Tamošiunas

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