High zone-selectivity of cell permeabilization following digitonin-pulse perfusion of rat liver. A re-interpretation of the microcirculatory zones

Histochemistry
B Quistorff, P Rømert

Abstract

The plasma membrane permeabilization obtained by exposure of hepatocytes to digitonin is utilized in the so-called digitonin-pulse perfusion of rat liver (Quistorff and Grunnet 1987). Brief pulses of digitonin applied with antegrade and retrograde perfusion of the liver caused selective elution of cytosolic enzymes and metabolites from the periportal and the perivenous zone of the same liver. In the present study a light microscopical examination of the liver fixed immediately after the digitonin pulse confirmed the very high zonal selectivity of the method inferred from the marker enzyme pattern of the eluates: Only cells around the port of entry of digitonin were affected and the borderline between affected and non-affected cells was always sharp. The typical periportal lesion was triangular in shape, enclosing the portal space, while the perivenous lesion was roughly circular, concentric with the hepatic vein. Assuming that the digitonin lesion reflects the microcirculatory flow pattern these findings seem to be at variance with the acinar model of Rappaport (Rappaport et al. 1954). The lesion in the lobuli near the surface of the liver as reflected by the discoloration pattern observed on the surface was the same as the les...Continue Reading

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Citations

Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H F TeutschE Groezinger
Aug 1, 1993·Cell and Tissue Research·K Wake, T Sato
Jul 6, 2000·The Biochemical Journal·S P BurnsR D Cohen
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Oct 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W H LamersA F Moorman

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