PMID: 42908Nov 1, 1979Paper

Higher molecular weight forms of immunoreactive somatostatin in mouse hypothalamic extracts: evidence of processing in vitro

Proceedings of the National Academy of Sciences of the United States of America
M LauberP Cohen

Abstract

Extracts of mouse hypothalamus made in acid/urea containing protease inhibitors were analyzed for somatostatin immunoreactivity after molecular sieve filtration on Sephadex G-50. Higher molecular weight (higher-M(r)) somatostatin-like forms with apparent molecular weights of 15,000, 10,000, and 6000 could be identified, besides the molecular weight 1600 somatostatin. Immunological identities with somatostatin were unambiguously demonstrated by the analysis of the displacement curves in the radioimmunoassay. The M(r) 15,000, 6000, and 1600 species were purified by affinity chromatography on an anti-somatostatin immune serum covalent conjugate with Sepharose used as immunoadsorbant. After disulfide reduction by dithiothreitol, the size of the M(r) 15,000 and 6000 somatostatin-like species was assessed either by molecular sieve filtration or by polyacrylamide gel electrophoresis. The results indicated that the higher-M(r) somatostatin-like species isolated from the hypothalamus did not result from hormone polymerization by means of disulfide interchange. The processing in vitro of the 15,000 higher-M(r) form of somatostatin was achieved by proteolytic enzymes coeluted with this species during the fractionation of hypothalamic extr...Continue Reading

References

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Citations

May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P NicolasP Cohen
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Shields
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R GamseS E Leeman
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W L TaylorJ E Dixon
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T G Warren, D Shields
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M MagazinJ E Dixon
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M RavazzolaL Orci
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P GluschankofP Cohen
Nov 1, 1982·The Journal of Clinical Investigation·H H Zingg, Y C Patel
Oct 1, 1991·Journal of Neurochemistry·G BonannoM Raiteri
Nov 1, 1983·Clinics in Endocrinology and Metabolism·A Gómez-Pan, M D Rodriguez-Arnao
Nov 1, 1983·Clinics in Endocrinology and Metabolism·J A Wass
Dec 1, 1982·Journal of Neurochemistry·T Yamada, S Basinger
Jun 1, 1982·Bioscience Reports·R Corder, P J Lowry
Apr 29, 1980·Biochemical and Biophysical Research Communications·H H Zingg, Y C Patel
Jun 24, 1980·Biochemistry·J Spiess, W Vale
Nov 19, 1981·Nature·C B Srikant, Y C Patel

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