PMID: 8973574Dec 1, 1996Paper

Higher-plant medium- and short-chain acyl-CoA oxidases: identification, purification and characterization of two novel enzymes of eukaryotic peroxisomal beta-oxidation

The Biochemical Journal
M A HooksI Couée

Abstract

Medium- and short-chain acyl-CoA oxidases were identified in and subsequently purified from dark-grown maize plantlets. The oxidase showing preference for medium-chain fatty acyl-CoAs (C10-C14) was purified to homogeneity. The oxidase showing preference for short-chain fatty acyl-CoAs (C4-C8) was purified over 150-fold. Various catalytic properties confirmed these enzymes to be true acyl-CoA oxidases. They produced trans-2-enoyl-CoA and H2O2 from the saturated acyl-CoA, as verified by various independent assay techniques. They also exhibited FAD-dependent activity; i.e. removal of loosely bound FAD by gel filtration markedly reduced activity, which could be restored upon re-addition of FAD. They showed apparent Km values between 2 and 10 microM for the acyl-CoA substrate giving maximal activity, no activity with the corresponding free fatty acid, high pH optima (8.3-8.6) and a peroxisomal subcellular location. The medium-chain acyl-CoA oxidase was determined to be a monomeric protein with a molecular mass of 62 kDa. The short-chain acyl-CoA oxidase was shown to have a native molecular mass of 60 kDa, but exhibited a labile multimeric structure, as indicated by the elution of multiple peaks of activity during several chromatogra...Continue Reading

Citations

Apr 23, 1999·The Journal of Biological Chemistry·H HayashiM Nishimura
May 14, 2010·The Journal of Biological Chemistry·Valerie E PyeAnette Henriksen
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·V MittendorfY Poirier
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M A HooksI A Graham
Jan 5, 2002·Progress in Lipid Research·Ian A Graham, Peter J Eastmond
Jun 20, 2000·Plant Physiology·B E FromanK Dehesh
Feb 15, 2008·Plant Physiology and Biochemistry : PPB·Susan ArentAnette Henriksen

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