PMID: 11604174Oct 18, 2001Paper

Higher production of rabies virus in serum-free medium cell cultures on microcarriers

Journal of Biotechnology
N M Frazzati-GallinaC A Pereira

Abstract

Rabies virus suspensions were obtained from VERO cells cultivated on solid microcarriers in a bioreactor after infection with the Pasteur rabies virus strain (PV). Virus production-serum free medium (VP-SFM) or Leibovitz 15 (L15) medium supplemented or not with fetal calf serum (FCS) were used to cultivate the VERO cells, before and after virus infection. The cell growth was shown to reach higher densities (1.6 x 10(6) cellsmol(-l)), when VP-SFM supplemented with 1% of FCS was used during the cell growth phase of culture, and then replaced by VP-SFM alone for the virus multiplication phase. In the cultures performed from the beginning with VP-SFM, lower densities accompanied by an altered cell morphology and detachment from the microcarriers were always observed. In rabies virus infected cultures, kinetic studies showed that higher virus yields (10(4.7) FFD(50) per 0.05 ml) were always obtained in cultures performed initially on VP-SFM supplemented with 1% FCS and after infection on VP-SFM alone. In agreement with that, rabies virus production, as measured by the average of virus titers in harvests obtained at different times after infection were shown to be 5.5 times higher in the cell cultures using initially VP-SFM+1%FCS and...Continue Reading

References

May 1, 1997·Journal of Industrial Microbiology & Biotechnology·O Pérez, C C Paolazzi
Feb 25, 1999·Revista Do Instituto De Medicina Tropical De São Paulo·N M GallinaR M Fuches

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Citations

Sep 22, 2009·The Journal of Infectious Diseases·Jonathan CennaJames P McGettigan
Feb 24, 2010·Antimicrobial Agents and Chemotherapy·Daniel AielloDonald T Moir
Sep 10, 2011·Pakistan Journal of Biological Sciences : PJBS·S Mehdi HassanzadehAhmad Fayaz
Jun 30, 2009·Vaccine·Cristian PailletMarina Etcheverrigaray
May 27, 2004·Biotechnology and Bioengineering·Olivier HenryAmine Kamen
Feb 5, 2004·Biotechnology and Bioengineering·A Y YokomizoC A Pereira
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·G A SilvaR L Reis
Jan 30, 2015·Biotechnology Progress·Laís Hara BuennoEutimio Gustavo Fernández Núñez
Feb 12, 2011·Journal of Proteomics·Monika Adamczyk-PoplawskaElzbieta K Jagusztyn-Krynicka
Jul 17, 2015·Expert Review of Vaccines·Lilí Esmeralda Gallo-RamírezUdo Reichl
Mar 13, 2012·Integrative Zoology·Sarah N BevinsHongxuan He

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