PMID: 3755738Jan 1, 1986Paper

Higher pyrimidine dimer yields in skin of normal humans with higher UVB sensitivity

The Journal of Investigative Dermatology
S E FreemanB M Sutherland

Abstract

We have measured UVB (280-320 nm)-induced DNA damage in skin of individuals with different sensitivities to UVB irradiation as measured by minimal erythema dose (MED). The DNA damage was susceptible to cleavage by Micrococcus luteus UV endonuclease, which recognizes pyrimidine dimers in DNA. An alkaline agarose gel electrophoresis method was used to quantitate the number of M. luteus UV endonuclease-sensitive sites in nonradioactive DNA from skin biopsies of 7 individuals irradiated with UVB (0-180 mJ X cm-2). The production of sites correlated well with MED (correlation coefficient = 0.78). The slope of the dose response curve for the most UVB-sensitive individual (MED = 24 mJ X cm-2) and for the least UVB-sensitive individual (MED = 146 mJ X cm-2) were 11.5 X 10(-4) and 2.6 X 10(-4) sites per 1000 bases per mJ X cm-2, respectively. The UVB-induced DNA damage was determined to be pyrimidine dimers by its susceptibility to cleavage by M. luteus UV endonuclease and its photoreactivability by Escherichia coli photoreactivating enzyme.

Citations

Mar 1, 1988·Photochemistry and Photobiology·S ChewL C Harber
Jun 1, 1986·Photochemistry and Photobiology·R W Gange, C F Rosen
Feb 3, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Damilola FajuyigbeAntony R Young
Aug 27, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Nicole SchadeJean Krutmann
Sep 1, 1986·Photochemistry and Photobiology·B M SutherlandJ C Sutherland
Feb 5, 2010·Pigment Cell & Melanoma Research·Zalfa A Abdel-MalekViki B Swope
Aug 1, 1987·Photochemistry and Photobiology·S E FreemanB M Sutherland
Aug 27, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Takeshi HorioHiroyuki Okamoto
Jan 1, 1988·Photochemistry and Photobiology·S E FreemanR D Ley
Jul 14, 2006·Photochemistry and Photobiology·Takeshi HorioHiroyuki Okamoto
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole RockelJean Krutmann
Dec 1, 1996·Critical Reviews in Biochemistry and Molecular Biology·S Beissert, R D Granstein
Jul 28, 2016·Pigment Cell & Melanoma Research·Damilola Fajuyigbe, Antony R Young
Aug 25, 1993·Journal of Chromatography·E A Ribeiro

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