Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis

The American Journal of Tropical Medicine and Hygiene
Fred Luciano Neves SantosYara Miranda Gomes

Abstract

The existence of an imperfect reference standard presents complications when evaluating the unbiased performance of novel diagnostic techniques. This is especially true in the absence of a gold standard, as is the case in chronic Chagas disease (CD) diagnosis. To circumvent this constraint, we elected to use latent class analysis (LCA). Previously, our group demonstrated the high performance of four Trypanosoma cruzi-chimeric proteins (Molecular Biology Institute of Paraná [IBMP]-8.1, -8.2, -8.3, and -8.4) for CD diagnosis using several distinct immunoassays. Although commercial tests had previously been established as a reference standard, the diagnostic performance of these chimeric antigens could present bias because these tests fail to produce 100% accurate results. Thus, we used LCA to assess the performance of these IBMP chimeric antigens in chronic CD diagnosis. Using the LCA model as a gold standard, sensitivity and specificity values ranged from 93.5% to 99.4% and 99.6% to 100%, respectively. The accuracy values were 96.2% for IBMP-8.2, approximately 98% for IBMP-8.1 and IBMP-8.3, and nearly 100% for IBMP-8.4. For IBMP-8.1 and IBMP-8.2, higher positive predictive values were associated with increases in hypothetical pr...Continue Reading

References

Apr 16, 1999·Journal of Clinical Microbiology·E S UmezawaJ F da Silveira
Jul 14, 1999·Tropical Medicine & International Health : TM & IH·M BoelaertP Van der Stuyft
Aug 4, 2009·Acta Tropica·Joaquim GasconMaría-Jesús Pinazo
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Yara M GomesAlejandro O Luquetti
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Leonardo FotiMarco Aurélio Krieger
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Walderez Ornelas DutraKenneth J Gollob
Nov 26, 2009·Acta Tropica·Gabriel A Schmunis, Zaida E Yadon
Aug 10, 2010·Academic Radiology·Rachel GoldPina C Sanelli
Oct 7, 2011·Clinical Microbiology Reviews·Caryn BernSusan P Montgomery
May 29, 2012·Infectious Disease Clinics of North America·Anis RassiJoffre Marcondes de Rezende
Jun 6, 2012·PLoS Neglected Tropical Diseases·Peter J HotezMaria Elena Bottazzi
Oct 22, 2013·Acta Tropica·Francisco Rogerlândio Martins-MeloJorg Heukelbach
Dec 5, 2013·Tropical Medicine & International Health : TM & IH·Yves JacksonSarah Pett
Jan 1, 2014·Emerging Infectious Diseases·Kazuo ImaiSachio Miura
Apr 9, 2014·The American Journal of Tropical Medicine and Hygiene·Diana L MartinCaryn Bern
May 20, 2015·The American Journal of Tropical Medicine and Hygiene·Jennifer Manne-GoehlerVeronika J Wirtz
Sep 27, 2015·Global Heart·Jeffrey D Stanaway, Gregory Roth
Jan 19, 2016·Acta Tropica·Erin E ConnersKimberly C Brouwer
Mar 16, 2016·The American Journal of Tropical Medicine and Hygiene·Fred Luciano Neves SantosYara de Miranda Gomes
Dec 17, 2016·Revista Da Sociedade Brasileira De Medicina Tropical·João Carlos Pinto DiasRenato Vieira Alves
Mar 9, 2017·PLoS Neglected Tropical Diseases·Fred Luciano Neves SantosYara de Miranda Gomes
Jul 21, 2017·Journal of Clinical Microbiology·Fred Luciano Neves SantosMarco Aurélio Krieger

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Citations

Mar 16, 2019·Journal of Clinical Pathology·Marco Vincenzo Lenti, Gino Roberto Corazza
Jun 27, 2019·PLoS Neglected Tropical Diseases·Leonardo M LeonyFred L N Santos
Aug 27, 2021·Biosensors·Paola Alejandra Fiorani CeledonFred Luciano Neves Santos

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
ELISA
protein assay

Software Mentioned

IBMP
STATA
Mplus

Related Concepts