Highly active antiretroviral therapy potently suppresses HIV infection in humanized Rag2-/-gammac-/- mice.

AIDS Research and Human Retroviruses
Kaori SangoHarris Goldstein

Abstract

Humanized Rag2(-/-)gamma(c)(-/-) mice (Hu-DKO mice) become populated with functional human T cells, B cells, and dendritic cells following transplantation with human hematopoietic stem cells (HSC) and represent an improved model for studying HIV infection in vivo. In the current study we demonstrated that intrasplenic inoculation of hu-DKO mice with HIV-1 initiated a higher level of HIV infection than intravenous or intraperitoneal inoculation, associated with a reciprocal decrease in peripheral CD4(+) T cells and increase in peripheral CD8(+) T cells. HIV infection by intrasplenic injection increased serum levels of human IgG and IgM including human IgM and IgG specific for HIV-1 gp120. There was a significant inverse correlation between the level of HIV-1 infection and the extent of CD4(+) T cell depletion. Highly active antiretroviral therapy (HAART) initiated 1 week after HIV-1 inoculation markedly suppressed HIV-1 infection and prevented CD4(+) T cell depletion. Taken together, these findings demonstrate that intrasplenic injection of hu-DKO mice with HIV is a more efficient route of HIV infection than intravenous or intraperitoneal injection and generates increased infection associated with an increased anti-HIV humoral r...Continue Reading

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Citations

Jan 10, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Santhi GorantlaLarisa Y Poluektova
Jan 20, 2009·Current HIV/AIDS Reports·Paul W Denton, J Victor Garcia
Apr 17, 2012·Cellular & Molecular Immunology·Liguo Zhang, Lishan Su
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Oct 21, 2011·Journal of Virology·Shailesh K ChoudharyDavid M Margolis
Oct 29, 2014·Expert Opinion on Drug Discovery·Katja Sliva
Nov 14, 2015·AIDS Research and Human Retroviruses·Kang Chang KimYoung Bong Kim
Jun 1, 2012·The Journal of General Virology·Nicolas LegrandMireille Centlivre
Feb 25, 2018·The Journal of Pharmacology and Experimental Therapeutics·JoEllyn M McMillanLarisa Y Poluektova
Dec 2, 2017·Current Opinion in HIV and AIDS·Guillemette Masse-RansonJames P Di Santo
Apr 20, 2017·Current Opinion in HIV and AIDS·Henning Gruell, Florian Klein

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Methods Mentioned

BETA
density gradient centrifugation
enzyme-linked immunosorbent assay
flow
FACS
FCS
ELISA
flow cytometry

Software Mentioned

Flowjo
Flowjo Analysis Program
SPSS

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