Highly altered protein expression profile in the adriamycin resistant MCF-7 cell line

Journal of Proteome Research
Marion L GehrmannYetrib Hathout

Abstract

The protein expression pattern in the cytosol fraction of the adriamycin resistant MCF-7 cell line (MCF-7/ADR) was compared to that of the parental MCF-7 cell line using two-dimensional gel electrophoresis and mass spectrometry. Twenty proteins with altered abundances were identified and studied in MCF-7/ADR. Both up regulation and down regulation are characterized. The most striking differences were found for proteins that were uniquely expressed in this cell line and not detectable in the parental MCF-7 cell line. These proteins include annexin I, the neuronal ubiquitin carboxyl hydrolase isoenzyme L-1 (also known as PGP9.5), glutathione-S-transferase pi class, nicotinamide N-methyltransferase, and interleukin-18 precursor. On the other hand, catechol-O-methyltransferase was expressed in the parental cell line, but was not detected in the adriamycin resistant cell line. This protein expression pattern was unique to MCF-7/ADR and not observed in MCF-7 cell lines selected for resistant to etoposide, mitoxantrone or melphalan.

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Citations

Feb 9, 2012·Breast Cancer Research and Treatment·Yukiko NakamuraShinzaburo Noguchi
Jun 9, 2004·Cancer Letters·Yetrib HathoutCatherine Fenselau
Dec 17, 2009·Omics : a Journal of Integrative Biology·Fengjia ZhuJiang Cao
Nov 22, 2007·Expert Opinion on Drug Metabolism & Toxicology·Robert O'ConnorLorraine O'Driscoll
Sep 15, 2007·Cancer Treatment Reviews·Jian-Ting Zhang, Yang Liu
Aug 1, 2009·Proteomics. Clinical Applications·Hermann Lage
Jun 6, 2006·Cancer·Laura SmithUNKNOWN Cancer Biology Proteomics Group
Sep 21, 2006·Rapid Communications in Mass Spectrometry : RCM·Hetal A SarvaiyaIulia M Lazar
Dec 12, 2012·Journal of Proteome Research·Jae-Sung YiYoung-Gyu Ko
Nov 25, 2011·Journal of Proteome Research·Adam R EvansRenã A S Robinson
Nov 17, 2010·Molecular and Cellular Biochemistry·Fengming GongAiping Tong
Jul 20, 2019·Molecular Cancer Research : MCR·Jorge Gómez-MiragayaEva González-Suárez
Sep 26, 2008·Journal of Proteome Research·Ye-Hong KuangTakuro Kanekura

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