Highly conserved type 1 pili promote enterotoxigenic E. coli pathogen-host interactions.

PLoS Neglected Tropical Diseases
Alaullah SheikhJ M Fleckenstein

Abstract

Enterotoxigenic Escherichia coli (ETEC), defined by their elaboration of heat-labile (LT) and/or heat-stable (ST) enterotoxins, are a common cause of diarrheal illness in developing countries. Efficient delivery of these toxins requires ETEC to engage target host enterocytes. This engagement is accomplished using a variety of pathovar-specific and conserved E. coli adhesin molecules as well as plasmid encoded colonization factors. Some of these adhesins undergo significant transcriptional modulation as ETEC encounter intestinal epithelia, perhaps suggesting that they cooperatively facilitate interaction with the host. Among genes significantly upregulated on cell contact are those encoding type 1 pili. We therefore investigated the role played by these pili in facilitating ETEC adhesion, and toxin delivery to model intestinal epithelia. We demonstrate that type 1 pili, encoded in the E. coli core genome, play an essential role in ETEC virulence, acting in concert with plasmid-encoded pathovar specific colonization factor (CF) fimbriae to promote optimal bacterial adhesion to cultured intestinal epithelium (CIE) and to epithelial monolayers differentiated from human small intestinal stem cells. Type 1 pili are tipped with the Fi...Continue Reading

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Citations

Mar 13, 2018·The Journal of Infectious Diseases·Jeffrey W BrownEsther Bullitt
May 18, 2018·The Journal of Clinical Investigation·Pardeep KumarJames M Fleckenstein
Aug 22, 2018·Infection and Immunity·Yuehui ZhuJames M Fleckenstein
Mar 5, 2019·Current Infectious Disease Reports·James M Fleckenstein, F Matthew Kuhlmann
Aug 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Alexander A CroftsM Stephen Trent
Jun 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Hiroya OkiShota Nakamura
Nov 13, 2020·Frontiers in Cellular and Infection Microbiology·Mario Meza-SeguraTeresa Estrada-Garcia
Nov 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Alaullah SheikhJames M Fleckenstein
May 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Joseph L BakerMagnus Andersson
Jul 18, 2021·The Journal of Infectious Diseases·James M Fleckenstein, Alaullah Sheikh

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Datasets Mentioned

BETA
CRL-2102

Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
Confocal microscopy
Scanning Confocal Microscopy
biopsies
PCR
size exclusion column chromatography
affinity purification
electrophoresis
fluorescence microscopy

Software Mentioned

NIS
ImageJ
Volocity
Elements
FlowJo
CellQuest

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