Highly effective antibacterial activity by the synergistic effect of three dimensional ordered mesoporous carbon-lysozyme composite

Journal of Colloid and Interface Science
Jingjing WangYang Shen

Abstract

Aiming at developing a safe and efficient alternative to traditional drinking water disinfection, this work successfully synthesized a novel antibacterial material with high surface area, ultra large pore size and tunable loading of immobilized lysozyme. The immobilized enzymes exhibit high antibacterial efficacy without forming carcinogenic disinfection byproducts. Critical immobilization parameters were optimized to keep the activity of the immobilized enzyme at a high level. The immobilization of lysozymes on 3DOm COOH could be confirmed by the characterizations of transmission electron microscopy, X-ray diffraction and Zeta-Potential. In addition, the structural stability of lysozymes on 3DOm COOH were studied by Fourier transform infrared spectroscopy. The antibacterial performance of 3DOm COOH-Lyz were specifically investigated based on the disinfection efficacy of Staphylococcus aureus in water. The results revealed that the immobilization capacity and relative activity of immobilized lysozyme were 814mg/g carrier and 80%, respectively, under the optimal immobilization conditions. And the antibacterial material with the initial mass ratio of lysozyme and 3DOm COOH as 3:1 exhibited maximum bacteria removal efficiency (98....Continue Reading

Citations

Aug 23, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Ericka Rodríguez-LeónJuan Manuel Martínez-Soto
Mar 23, 2019·Journal of Colloid and Interface Science·Youkun ZhengXuemei Wang

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