Highly efficient Agrobacterium-mediated transformation of Volvariella volvacea

Bioresource Technology
Jie WangJunfang Lin

Abstract

Agrobacterium tumefaciens-mediated transformation (ATMT) was successfully applied to the edible straw mushroom, Volvariella volvacea. Mycelium pellets were transformed to cold stress resistance using the afp gene as both a selective marker and a reporter gene, under the control of a heterologous Lentinula edodes gpd promoter. The efficiency of transformation is over 100 times higher than that previously reported in V. volvacea. Stable integration of the afp gene with 1-4 copy numbers was confirmed in all 10 randomly selected transgenic events by Southern blot analysis. The mitotic stability of the transformants was demonstrated after five successive transfers on PDA medium without selection pressure and the PCR analysis of basidiospores harvested from transformants.

References

Sep 22, 1998·Nature Biotechnology·M J de GrootA G Beijersbergen
May 21, 2003·Current Genetics·Vera MeyerUlf Stahl
Nov 25, 2003·Current Genetics·Andreas LeclerqueHans Ulrich Schairer
Jul 6, 2004·Journal of Microbiological Methods·Maria Cecília dos ReisMarcia Cristina Furlaneto

❮ Previous
Next ❯

Citations

Aug 22, 2008·Applied Microbiology and Biotechnology·Mingqian FengRenxiang Tan
May 31, 2013·Journal of Basic Microbiology·Lin-Feng YouHai-Xing Yang
Feb 7, 2015·Folia Microbiologica·Yu-Ping ZhouChang-En Tian
Mar 13, 2015·Molecular Biotechnology·LinZhi KangLiQiong Guo
Aug 13, 2013·Fungal Biology·Maria Beatriz Calderan RodriguesAline Aparecida Pizzirani-Kleiner
Oct 4, 2016·Biotechnology and Applied Biochemistry·Wei JiangXueming Tang
Apr 22, 2015·Mycobiology·Sinil KimHyeon-Su Ro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.