Highly efficient and selective enrichment of peptide subsets combining fluorous chemistry with reversed-phase chromatography.

Rapid Communications in Mass Spectrometry : RCM
Wantao YingMark E McComb

Abstract

The selective capture of target peptides poses a great challenge to modern chemists and biologists, especially when enriching them from proteome samples possessing extremes in concentration dynamic range and sequence diversity. While approaches based on traditional techniques such as biotin-avidin pairing offer versatile tools to design strategies for selective enrichment, problems are still encountered due to sample loss or poor selectivity of enrichment. Here we show that the recently introduced fluorous chemistry approach has attractive properties as an alternative method for selective enrichment. Through appending a perfluorine group to the target peptide, it is possible to dramatically increase the peptide's hydrophobicity and thus enable facile separation of labeled from non-labeled peptides. Use of reversed-phase chromatography allowed for improved peptide recovery in comparison with results obtained using the formerly reported fluorous bonded phase methods. Furthermore, this approach also allowed for on-line separation and identification of both labeled and unlabeled peptides in a single experiment. The net result is an increase in the confidence of protein identification by tandem mass spectrometry (MS2) as all peptide...Continue Reading

References

Apr 17, 2003·Journal of the American Chemical Society·Anna E SpeersBenjamin F Cravatt
Jan 17, 2004·Molecular & Cellular Proteomics : MCP·Mahadevan SethuramanRichard A Cohen
Jan 11, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Magnus Palmblad, John S Vogel
Mar 16, 2005·Nature Biotechnology·Scott M BrittainEric C Peters
Jul 13, 2005·Organic & Biomolecular Chemistry·Zoltán KaletaTibor Soós
Sep 22, 2005·Journal of the American Chemical Society·Kwang-Seuk KoNicola L Pohl
Jan 13, 2006·Nature Chemical Biology·Shao-En Ong, Matthias Mann
Oct 24, 2006·Current Opinion in Chemical Biology·Nicholas C YoderKrishna Kumar
Dec 1, 2006·Biochimica Et Biophysica Acta·Hildegard Geyer, Rudolf Geyer
Feb 22, 2007·Journal of Proteome Research·Carlos A GartnerSteven P Gygi
Mar 9, 2007·Journal of Proteome Research·Eden P GoGary Siuzdak
May 5, 2007·Analytical Chemistry·Jennifer L FrahmDavid C Muddimana
May 10, 2007·Nature Protocols·Tine E ThingholmMartin R Larsen
Jun 16, 2007·Molecular & Cellular Proteomics : MCP·Rebecca L CharlesPhilip Eaton
Jul 16, 2008·Journal of Proteomics·Xiangming Fang, Wei-Wei Zhang
Jan 17, 2009·Chemical Reviews·Wei Zhang

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Citations

May 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Tadashi HayamaHitoshi Nohta
Sep 10, 2014·Journal of Hematology & Oncology·Shanique EdwardsPing Xie
Dec 20, 2015·Proteomics·Andreas HentschelRobert Ahrends
Nov 28, 2013·Analytical Chemistry·Yuhuan JiCheng Lin
Jan 14, 2020·Bioconjugate Chemistry·Ivan BassaniniSergio Romeo
Mar 30, 2017·Analytical Chemistry·Cheng ZhangHaojie Lu

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