Highly efficient and selective isolation of rare tumor cells using a microfluidic chip with wavy-herringbone micro-patterned surfaces

The Analyst
Shunqiang WangYaling Liu

Abstract

Circulating tumor cells (CTCs) in peripheral blood have been recognized as a general biomarker for diagnosing cancer and providing guidance for personalized treatments. Yet due to their rarity, the challenge for their clinical utility lies in the efficient isolation while avoiding the capture of other non-targeted white blood cells (WBCs). In this paper, a wavy-herringbone (HB) microfluidic chip coated with antibody directly against epithelial cell adhesion molecule (anti-EpCAM) was developed for highly efficient and selective isolation of tumor cells from tumor cell-spiked whole blood samples. By extending the concept of the hallmark HB-Chip in the literature, the wavy-HB chip not only achieves high capture efficiency (up to 85.0%) by micro-vortexes induced by HB structures, but also achieves high purity (up to 39.4%) due to the smooth wavy microstructures. These smooth wavy-HB structures eliminate the ultra-low shear rate regions in the traditional grooved-HB structures that lead to non-specific trapping of cells. Compared with the grooved-HB chip with sharp corners, the wavy-HB chip shows significantly higher purity while maintaining similarly high capture efficiency. Furthermore, the wavy-HB chip has up to 11% higher captur...Continue Reading

References

Jan 26, 2002·Science·Abraham D StroockGeorge M Whitesides
Jul 16, 2002·Annual Review of Biomedical Engineering·David J BeebeGlenn M Walker
Feb 23, 2007·Cancer Letters·Patrizia Paterlini-Brechot, Naoual Linda Benali
May 4, 2007·Lab on a Chip·N Scott Lynn, David S Dandy
Jun 28, 2008·Lab on a Chip·Manda S WilliamsPaul Yager
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Johann S de BonoDerek Raghavan
Nov 22, 2008·Lab on a Chip·Chia-Hsien HsuMehmet Toner
Sep 22, 2010·International Journal of Cancer. Journal International Du Cancer·Michal MegoMassimo Cristofanilli
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shannon L StottMehmet Toner
Mar 17, 2011·Biomedical Microdevices·Cari A LauniereDavid T Eddington
Oct 19, 2011·The Analyst·Jana C JokerstCharles S Henry
Jun 5, 2013·Computers & Structures·Jifu TanYaling Liu
Jul 3, 2013·Methods : a Companion to Methods in Enzymology·Yi-Tsung LuEdwin M Posadas
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael J MitchellMichael R King
Jan 11, 2014·Lab on a Chip·Yuchao ChenTony Jun Huang
May 14, 2014·Nature Reviews. Clinical Oncology·David T MiyamotoRichard J Lee
Jul 26, 2014·Biosensors & Bioelectronics·Kyung-A HyunHyo-Il Jung

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Citations

Feb 18, 2017·ACS Applied Materials & Interfaces·Faheng ZangReza Ghodssi
Jun 21, 2017·Chemical Society Reviews·Joshua M JacksonSteven A Soper
Mar 28, 2019·Genetic Testing and Molecular Biomarkers·Matthew ScarlottaAmy K Kim
Feb 1, 2018·Proceedings of the Institution of Mechanical Engineers. Part C. Journal of Mechanical Engineering Science·Jifu TanYaling Liu
Sep 30, 2017·Electrophoresis·Ye ZhangDimitri Pappas
Jan 31, 2017·Annual Review of Pathology·J-A ThieleP Kuhn
May 26, 2018·The Analyst·Hyungseok ChoKi-Ho Han
Dec 28, 2018·Cancers·Marta Tellez-GabrielDominique Heymann
Mar 22, 2018·Biotechnology Advances·Sandhya SharmaWaseem Asghar
May 4, 2021·ACS Sensors·Thilanga N PahattugeSteven A Soper
Mar 21, 2018·ACS Applied Materials & Interfaces·Yaw Opoku-DamoahYang Ding
Jan 29, 2020·ACS Applied Materials & Interfaces·Xu A ZhangDaeyeon Lee

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