Abstract
The transduction of cerebellar neurons in vivo with herpes simplex virus 1 (HSV-1) amplicon carrying the lacZ gene has been investigated after injection of the vector in the cerebellar cortex, ventricles, and inferior olive of adult rats. Injection into the cerebellar cortex resulted in transduction of Purkinje cells near the needle tract and injection into the ventricles yielded no transduced neurons. In contrast, high transduction efficiency was achieved by vector injection into the inferior olive, resulting in one of three positive Purkinje cells all over the ipsilateral and contralateral cerebellar hemispheres. Because neurons in the deep cerebellar nuclei are also transduced, we suggest that the vector is delivered from the inferior olive to the cerebellar nuclei and then to Purkinje cells by retrograde axonal transport. Expression of the lacZ gene within Purkinje cells was surprisingly persistent and was maintained at the same level for at least 40 days. Importantly, no signs of either toxicity or inflammation were observed in the cerebellum after vector injection, except for the borders of the needle tract where some reactive astrocytes were detected. Indeed, motor coordination of treated animals was entirely normal, as ...Continue Reading
References
Oct 29, 1976·Brain Research·D Dahl, A Bignami
Jan 1, 1992·Virology·I L SmithR M Sandri-Goldin
Oct 1, 1991·Trends in Neurosciences·A I GellerR L Neve
May 1, 1989·Brain Research Bulletin·J H McLeanD I Bernstein
Jan 1, 1989·Journal of Virology·A M McCarthyP A Schaffer
Sep 23, 1988·Science·A I Geller, X O Breakefield
Jan 1, 1989·Journal of Neuroscience Research·M B GraeberG W Kreutzberg
Aug 1, 1982·Cell·R R Spaete, N Frenkel
Jan 28, 1994·Gene·R LyleJ E Hewitt
Jan 1, 1996·Nature Medicine·W ZhangL C Triarhou
Mar 1, 1996·BioTechniques·F LimA I Geller
May 15, 1996·Journal of Neuroscience Research·M Araujo, F Wandosell
Jan 20, 1996·Human Gene Therapy·M HashimotoK Mikoshiba
Oct 1, 1996·Journal of Virology·C FraefelA I Geller
Dec 24, 1997·Anatomy and Embryology·T TerashimaH Okado
Jul 8, 1998·Progress in Neurobiology·W T Hermens, J Verhaagen
Nov 11, 1998·Neuroscience and Biobehavioral Reviews·R B Norgren, M N Lehman
May 29, 1999·Journal of Neuroscience Research·J R Muñoz-MontañoJ Díaz-Nido
Jul 22, 1999·Journal of Neurovirology·J J GarridoT Schimmang
Jul 23, 1999·Experimental Neurology·W F Kaemmerer, W C Low
Sep 16, 1999·Experimental Neurology·D WolfeJ C Glorioso
Oct 30, 1999·Human Gene Therapy·L C CostantiniO Isacson
Apr 27, 2000·Trends in Neurosciences·M SimonatoJ Glorioso
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·C A Strathdee, M R McLeod
Sep 8, 2000·Neuroreport·J M AliskyB L Davidson
Nov 18, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·W F KaemmererW C Low
Dec 13, 2000·Brain Research. Molecular Brain Research·G R ZhangA I Geller
Aug 23, 2001·BioTechniques·P de FelipeF Lim
Citations
Apr 18, 2008·The Cerebellum·Hirokazu Hirai
Apr 15, 2011·Gene Therapy·A Gimenez-CassinaJ Diaz-Nido
Jul 17, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michele M P LufinoRichard Wade-Martins
Jan 20, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Silvia Gomez-SebastianRichard Wade-Martins
Mar 22, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Filip LimJavier Díaz-Nido
Sep 24, 2010·Journal of Biomedicine & Biotechnology·Sara Pérez-Luz, Javier Díaz-Nido
Aug 12, 2010·The Cerebellum·Jean-Pierre LouboutinDavid S Strayer
May 9, 2006·Developmental Neuroscience·C CrociE R Bongarzone
Aug 2, 2007·Expert Opinion on Biological Therapy·Delphine CuchetAlberto L Epstein
Jul 15, 2005·Journal of Neuroscience Research·C A PaulR A Maue
Jul 14, 2006·The European Journal of Neuroscience·Takashi TorashimaHirokazu Hirai
Jun 28, 2006·Journal of Neuroscience Research·Alfredo Gimenez-CassinaJavier Diaz-Nido
Jul 11, 2012·The European Journal of Neuroscience·Jun NishiyamaMichisuke Yuzaki
Sep 23, 2006·Neurochemistry International·Alfredo Gimenez-CassinaJavier Diaz-Nido
Jul 1, 2006·Neuroscience·M L CortésX O Breakefield
Mar 7, 2006·Brain Research·Takashi TorashimaHirokazu Hirai
Jun 2, 2015·Genomics·S Pérez-LuzJ Díaz-Nido
Sep 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Angelika OehmigXandra O Breakefield
Dec 19, 2003·Gene Therapy·J QiS Wang
Oct 13, 2009·Neurochemical Research·Masahiko Tanaka