Highly efficient and ultra-small volume separation by pressure-driven liquid chromatography in extended nanochannels

Small
Ryo IshibashiTakehiko Kitamori

Abstract

The rapidly developing interest in nanofluidic analysis, which is used to examine liquids ranging in amounts from the attoliter to the femtoliter scale, correlates with the recent interest in decreased sample amounts, such as in the field of single-cell analysis. For general nanofluidic analysis, the fact that a pressure-driven flow does not limit the choice of solvents (aqueous or organic) is important. This study shows the first pressure-driven liquid chromatography technique that enables separation of atto- to femtoliter sample volumes, with a high separation efficiency within a few seconds. The apparent diffusion coefficient measurement of the unretentive sample suggests that there is no increase in the viscosity of toluene in the extended nanospace, unlike in aqueous solvents. Evaluation of the normal phase separation, therefore, should involve only the examination of the effect of the small size of the extended nanospace. Compared to a conventionally packed high-performance liquid chromatography column, the separation here results in a faster separation (4 s) by 2 orders of magnitude, a smaller injection volume (10(0) fL) by 9 orders, and a higher separation efficiency (440,000 plates/m) by 1 order. Moreover, the separati...Continue Reading

References

Dec 14, 2002·Electrophoresis·Marja-Liisa Riekkola
Dec 25, 2002·Journal of Chromatography. a·D ClicG Desmet
Jul 2, 2003·Journal of Chromatography. a·H Poppe
Mar 17, 2004·Analytical Chemistry·Mathieu FoquetHarold G Craighead
Apr 23, 2004·Current Opinion in Biotechnology·Helene Andersson, Albert van den Berg
Nov 1, 2005·Analytical Chemistry·Sumita Pennathur, Juan G Santiago
Nov 1, 2005·Journal of Chromatography. a·Sarah VankrunkelsvenGert Desmet
May 26, 2006·Analytical and Bioanalytical Chemistry·Noritada KajiYoshinobu Baba
Oct 27, 2006·Lab on a Chip·Sergio L S Freire, Aaron R Wheeler
Mar 14, 2007·Angewandte Chemie·Takehiko TsukaharaTakehiko Kitamori
Jul 12, 2007·Journal of Separation Science·Hamed Eghbali, Gert Desmet
Jun 14, 2008·Journal of Chromatography. a·Xiayan WangShaorong Liu
Jun 27, 2008·Analytical and Bioanalytical Chemistry·Takehiko TsukaharaTakehiko Kitamori
Oct 31, 2008·Talanta·Christopher T CulbertsonJ Michael Ramsey
Jan 6, 2009·Journal of Chromatography. a·Akihide HibaraTakehiko Kitamori
Aug 12, 2009·Analytical Chemistry·Michelle L Kovarik, Stephen C Jacobson
Sep 2, 2009·Electrophoresis·Takehiko TsukaharaTakehiko Kitamori
Dec 17, 2009·Analytical Chemistry·Changbei Ma, Edward S Yeung
Dec 25, 2009·Analytical Chemistry·Masaru KatoTakehiko Kitamori
Feb 20, 2010·Current Opinion in Biotechnology·Andreas SchmidLars M Blank
Mar 9, 2011·Chemistry : a European Journal·Tsuyoshi TaniguchiHiroyuki Ishibashi

❮ Previous
Next ❯

Citations

Dec 12, 2012·Analytical Chemistry·Kazuma MawatariTakehiko Kitamori
Apr 17, 2012·Journal of Chromatography. a·Ryo IshibashiTakehiko Kitamori
Dec 18, 2013·Small·Kentaro ShiraiTakehiko Kitamori
Oct 3, 2015·Journal of Chromatography. a·Adelina SmirnovaTakehiko Kitamori
Aug 17, 2016·The Analyst·Hisashi ShimizuTakehiko Kitamori
Sep 14, 2016·Journal of Chromatography. a·Hisashi ShimizuTakehiko Kitamori
Sep 20, 2016·Analytical Chemistry·Adelina SmirnovaTakehiko Kitamori
Mar 30, 2018·Lab on a Chip·Kazuma MawatariTakehiko Kitamori
Apr 4, 2019·Lab on a Chip·Yutaka KazoeTakehiko Kitamori
Feb 13, 2020·The Analyst·Tatsuro NakaoTakehiko Kitamori
Nov 13, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Adelina SmirnovaTakehiko Kitamori
Jan 13, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ling LinTakehiko Kitamori
Sep 27, 2020·Micromachines·Thu Hac Huong LeKyojiro Morikawa
Oct 31, 2020·Analytical Chemistry·Koki YamamotoYo Tanaka
Nov 5, 2014·Analytical Chemistry·Daniel G HaywoodStephen C Jacobson
Jan 22, 2019·Analytical Chemistry·Hisashi ShimizuTakehiko Kitamori
Jul 25, 2019·Analytical Chemistry·Yoshiyuki Tsuyama, Kazuma Mawatari
Jun 1, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroki SanoTakehiko Kitamori
Nov 18, 2021·The Analyst·Takayuki KawaiKoji Otsuka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.