Highly efficient capture and harvest of circulating tumor cells on a microfluidic chip integrated with herringbone and micropost arrays

Biomedical Microdevices
Peng XueYuejun Kang

Abstract

Circulating tumor cells (CTCs), which are derived from primary tumor site and transported to distant organs, are considered as the major cause of metastasis. So far, various techniques have been applied for CTC isolation and enumeration. However, there exists great demand to improve the sensitivity of CTC capture, and it remains challenging to elute the cells efficiently from device for further biomolecular and cellular analyses. In this study, we fabricate a dual functional chip integrated with herringbone structure and micropost array to achieve CTC capture and elution through EpCAM-based immunoreaction. Hep3B tumor cell line is selected as the model of CTCs for processing using this device. The results demonstrate that the capture limit of Hep3B cells can reach up to 10 cells (per mL of sample volume) with capture efficiency of 80% on average. Moreover, the elution rate of the captured Hep3B cells can reach up to 69.4% on average for cell number ranging from 1 to 100. These results demonstrate that this device exhibits dual functions with considerably high capture rate and elution rate, indicating its promising capability for cancer diagnosis and therapeutics.

References

Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·F F BeckerP R Gascoyne
Nov 2, 2002·Electrophoresis·Jessamine M K NgGeorge M Whitesides
Aug 8, 2006·Nature Medicine·Larry Norton, Joan Massagué
Nov 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·G Thomas BuddDaniel F Hayes
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Apr 24, 2009·Biomedical Microdevices·Swee Jin TanChwee Teck Lim
Jan 13, 2010·Cell·Mi-Young KimJoan Massagué
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shannon L StottMehmet Toner
Oct 20, 2010·Nature Reviews. Clinical Oncology·Michal MegoMassimo Cristofanilli
Oct 28, 2010·Biomedical Microdevices·Siyang ZhengYu-Chong Tai
Jan 6, 2011·Lab on a Chip·Jaap den Toonder
Jun 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daniel C DanilaHoward I Scher
Aug 9, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·L A DevrieseJ H M Schellens
Aug 25, 2011·Lab on a Chip·Kazunori HoshinoXiaojing Zhang
Sep 8, 2011·Cancer Research·Anja van de StolpeJaap M J den Toonder
Sep 16, 2011·Biomicrofluidics·Soojung Claire HurDino Di Carlo
Apr 25, 2012·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Anieta M Sieuwerts, Stefanie S Jeffrey
Oct 20, 2012·Lab on a Chip·Jason G KraljSamuel P Forry
Apr 30, 2013·Lab on a Chip·Sunil K AryaAbdur Rub Abdur Rahman
Apr 4, 2014·BioMed Research International·Osamu KimuraTooru Shimosegawa

❮ Previous
Next ❯

Citations

Oct 14, 2016·Nucleic Acid Therapeutics·Eman M HassanMaria C DeRosa
Jan 14, 2017·Physical Review. E·Eduard Benet, Franck J Vernerey
Feb 6, 2020·Electrophoresis·Ye Tian, Liqiu Wang
Mar 4, 2021·Cancers·Amelia J RushtonR Charles Coombes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.