Highly efficient Cas9-mediated transcriptional programming

Nature Methods
Alejandro ChavezGeorge M Church

Abstract

The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).

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Citations

Sep 26, 2015·International Journal of Molecular Sciences·Toshitsugu Fujita, Hodaka Fujii
Feb 13, 2016·Genetics Research·Peng LiuShaohua Xu
Feb 7, 2016·Nucleic Acids Research·Sanjay GhoshJi-Long Liu
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Methods Mentioned

BETA
transfection
flow
flow cytometry

Software Mentioned

Matlab scripts
GraphPad Prism
iNeuron
Fiji
Matlab
custom Fiji
SunTag
Zen Blue
SAM
SUperNova

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