Dec 20, 2014

Production of Hyaluronan by the Trophectoderm is a Prerequisite for Mouse Blastocyst Attachment

BioRxiv : the Preprint Server for Biology
Alejandro ChavezGeorge M Church

Abstract

Successful embryo implantation requires the execution of highly synchronized processes at the feto-maternal interface, initiated by blastocyst attachment to the endometrial epithelium. Hyaluronan is a major ECM component known to regulate adhesion-associated biological processes in various physiological settings. We hypothesized that the adhesive properties of hyaluronan may facilitate blastocyst attachment. In order to test our hypothesis, we characterized the blastocyst expression of hyaluronan synthesizing and degrading enzymes, as well as the expression of hyaluronan receptors during attachment. The functional impact of hyaluronan was further challenged by the use of mouse transgenic blastocysts, in which genes encoding for hyaluronan synthesizing enzymes were deleted using lentiviral incorporation of Cas-9 endonuclease alongside specific short-guide RNAs into the embryonic trophectoderm. Embryos with transgenic trophectoderm were either tested for their attachment in vitro, or assessed for successful implantation in vivo, upon transfer to foster dams. Deletion of the trophectoderm hyaluronan biosynthesis significantly reduced the number of blastocysts attached to human uterine epithelium cells in vitro. A reduced attachmen...Continue Reading

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Mentioned in this Paper

Neuron Differentiation
Transcriptional Regulation
Pluripotent Stem Cells
Exons
Genes
Transcription, Genetic
Alzheimer's Disease
Cell Differentiation Process
Synaptotagmin p65
NEUROD1

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