Highly efficient conversion of xylose to ethanol without glucose repression by newly isolated thermotolerant Spathaspora passalidarum CMUWF1-2

BMC Microbiology
Nadchanok RodrussameeMamoru Yamada

Abstract

Efficient bioconversion of lignocellulosic biomass to bioethanol is one of key challenges in the situation of increasing bioethanol demand. The ethanologenic microbes for such conversion are required to possess abilities of utilization of various sugars including xylose and arabinose in lignocellulosic biomass. As required additional characteristics, there are a weak or no glucose repression that allows cells to simultaneously utilize various sugars together with glucose and thermotolerance for fermentation at high temperatures, which has several advantages including reduction of cooling cost. Spathaspora passalidarum ATCC MYA-4345, a type strains, isolated previously have mainly of these abilities or characteristics but its thermotolerance is not so strong and its glucose repression on xylose utilization is revealed. Newly isolated S. passalidarum CMUWF1-2 was found to have a high ability to produce ethanol from various sugars included in lignocellulosic biomass at high temperatures. The strain achieved ethanol yields of 0.43 g, 0.40 g and 0.20 g ethanol/g xylose at 30 °C, 37 °C and 40 °C, respectively. Interestingly, no significant glucose repression was observed in experiments with mixed sugars, being consistent with the str...Continue Reading

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Citations

Feb 7, 2019·Frontiers in Microbiology·Meirong GaoZengyi Shao
Nov 30, 2019·Applied Microbiology and Biotechnology·Mochamad NurcholisMamoru Yamada
Dec 15, 2020·FEMS Microbiology Reviews·Justyna Ruchala, Andriy A Sibirny
Mar 9, 2021·Applied Biochemistry and Biotechnology·Thályta F PachecoSílvia B Gonçalves

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Datasets Mentioned

BETA
LC062703

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
MEGA7

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