Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling.

Proceedings of the National Academy of Sciences of the United States of America
David C HayJohn P Iredale

Abstract

Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. To date, however, their homogeneous cellular differentiation to specific cell types in vitro has proven difficult. Wnt signaling has been shown to play important roles in coordinating development, and we demonstrate that Wnt3a is differentially expressed at critical stages of human liver development in vivo. The essential role of Wnt3a in hepatocyte differentiation from hESCs is paralleled by our in vitro model, demonstrating the importance of a physiologic approach to cellular differentiation. Our studies provide compelling evidence that Wnt3a signaling is important for coordinated hepatocellular function in vitro and in vivo. In addition, we demonstrate that Wnt3a facilitates clonal plating of hESCs exhibiting functional hepatic differentiation. These studies represent an important step toward the use of hESC-derived hepatocytes in high-throughput metabolic analysis of human liver function.

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P M HollandD H Gelfand
Oct 1, 1984·Archives of Toxicology·A M MitchellC R Elcombe
Nov 6, 1998·Science·J A ThomsonJ M Jones
Aug 4, 1999·Nature Genetics·P LiuA Bradley
Jan 5, 2000·Genes & Development·T P YamaguchiA P McMahon
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso
Apr 27, 2000·Mechanisms of Development·Y SakaJ C Smith
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SchuldinerN Benvenisty
Sep 5, 2001·Current Opinion in Genetics & Development·K S Zaret
Apr 16, 2003·Cell Transplantation·Lakshmi RambhatlaMelissa K Carpenter
Jul 5, 2003·Genes & Development·Stephane D VincentElizabeth J Robertson
Dec 13, 2003·Biological Chemistry·Jaroslaw CzyzAnna M Wobus
Jan 15, 2004·Experimental Cell Research·Sunny Z HussainSatdarshan P S Monga
Jul 24, 2004·Differentiation; Research in Biological Diversity·Neta LavonNissim Benvenisty
Oct 7, 2004·Journal of Hepatology·Céline FilippiJohn N Plevris
Aug 16, 2005·Cell Calcium·Aimee D Kohn, Randall T Moon
Nov 1, 2005·Nature Biotechnology·Kevin A D'AmourEmmanuel E Baetge
Jan 31, 2006·Journal of Cell Science·Ken M Cadigan, Yan I Liu
Jun 6, 2006·Genes & Development·Karl Willert, Katherine A Jones
Oct 21, 2006·Nature Biotechnology·Kevin A D'AmourEmmanuel E Baetge
Nov 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Paul GadueGordon M Keller
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yu LiuMichael D Schneider
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun CaiHongkui Deng

❮ Previous
Next ❯

Citations

Jun 21, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Allen Wang, Maike Sander
Jan 13, 2011·Archives of Toxicology·Anna M Wobus, Peter Löser
Oct 20, 2010·Cell and Tissue Research·Caroline Beth Sangan, David Tosh
Apr 5, 2011·Journal of Materials Science. Materials in Medicine·Andreas K NusslerThomas S Weiss
Jan 27, 2009·Current Gastroenterology Reports·Norman L SussmanJames H Kelly
Mar 1, 2012·Archives of Pharmacal Research·Xiao-Ling ZhouIn-Hyun Park
Sep 14, 2012·Stem Cells and Development·Louise SivertssonMagnus Ingelman-Sundberg
Jul 21, 2011·British Medical Bulletin·Massoud VosoughHossein Baharvand
Sep 13, 2012·American Journal of Physiology. Endocrinology and Metabolism·Wilfred IpTianru Jin
Apr 17, 2012·Journal of Cell Science·Wenli ZhouDavid C Hay
Jun 10, 2011·Proceedings of the American Thoracic Society·Daniel J WeissDarwin J Prockop
Jan 1, 2009·Critical Reviews in Biomedical Engineering·Hiroshi YagiAlejandro Soto-Gutierrez
Mar 11, 2009·The Journal of Endocrinology·Yin Xia, Alan L Schneyer
Mar 5, 2010·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Blaine W Phillips, Jeremy M Crook
Nov 10, 2013·International Journal of Molecular Sciences·Pingnan SunDavid C Hay
May 9, 2012·World Journal of Gastroenterology : WJG·Wen-Li ZhouDavid C Hay
Nov 9, 2011·Journal of Visualized Experiments : JoVE·Claire N MedineDavid C Hay
Jul 8, 2009·Pakistan Journal of Biological Sciences : PJBS·Mahmoud Hashemi-TabarFereshteh Negad Dehbashi
May 18, 2013·Stem Cells Translational Medicine·Xiaocui MaMark A Zern
Jan 9, 2013·Annual Review of Pharmacology and Toxicology·Dagmara SzkolnickaDavid C Hay

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.