Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

Applied Microbiology and Biotechnology
Hu ZengYuhui Sun

Abstract

The current diminishing returns in finding useful antibiotics and the occurrence of drug-resistant bacteria call for the need to find new antibiotics. Moreover, the whole genome sequencing revealed that the biosynthetic potential of Streptomyces, which has produced the highest numbers of approved and clinical-trial drugs, has been greatly underestimated. Considering the known gene editing toolkits were arduous and inefficient, novel and efficient gene editing system are desirable. Here, we developed an engineered CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein) combined with the counterselection system CodA(sm), the D314A mutant of cytosine deaminase, to rapidly and effectively edit Streptomyces genomes. In-frame deletion of the actinorhodin polyketide chain length factor gene actI-ORF2 was created in Streptomyces coelicolor M145 as an illustration. This CRISPR/Cas9-CodA(sm) combined system strikingly increased the frequency of unmarked mutants and shortened the time required to generate them. We foresee the system becoming a routine laboratory technique for genome editing to exploit the great biosynthetic potential of Streptomyces and perhaps for other medically and economically ...Continue Reading

References

Jan 1, 1992·Applied Microbiology and Biotechnology·T Schmitt-John, J W Engels
Oct 1, 1988·Molecular & General Genetics : MGG·Z X DengD A Hopwood
Jan 1, 1993·Annual Review of Microbiology·K F Chater
Oct 9, 2001·Nature Medicine·O Smithies
Apr 27, 2002·Applied and Environmental Microbiology·Nathalie CampoPascal Le Bourgeois
Sep 7, 2002·Science·Geoffrey R WellerAidan J Doherty
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertolt GustKeith F Chater
Mar 22, 2005·Nature Structural & Molecular Biology·Chunling GongMichael S Glickman
Apr 9, 2005·The Journal of Antibiotics·János Bérdy
Jun 6, 2008·Current Opinion in Pharmacology·Richard H Baltz
Jan 9, 2010·Science·Philippe Horvath, Rodolphe Barrangou
May 18, 2010·Applied Microbiology and Biotechnology·Theresa SieglAndriy Luzhetskyy
Jun 10, 2010·Annual Review of Microbiology·Hélène DeveauSylvain Moineau
May 10, 2011·Nature Reviews. Microbiology·Kira S MakarovaEugene V Koonin
Jun 28, 2011·Acta Biochimica Et Biophysica Sinica·Peng GuoZhongjun Qin
Nov 9, 2011·Annual Review of Genetics·Devaki BhayaRodolphe Barrangou
Jan 17, 2012·Applied and Environmental Microbiology·Simone HerrmannAndriy Luzhetskyy
Feb 22, 2012·Journal of Industrial Microbiology & Biotechnology·Xiaojuan ZhangJilun Li
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 31, 2013·Nature Biotechnology·Woong Y HwangJ Keith Joung
Jun 25, 2013·Nature Biotechnology·Yanfang FuJeffry D Sander
Jul 23, 2013·Nature Biotechnology·Patrick D HsuFeng Zhang
Aug 10, 2013·Nature Biotechnology·Qiwei ShanCaixia Gao
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xingjie RenJian-Quan Ni
Apr 15, 2014·Bioinformatics·An XiaoBo Zhang
Feb 18, 2014·Cell·Hiroshi NishimasuOsamu Nureki
Jun 2, 2014·Nature Biotechnology·Mehdi GhorbalJose-Juan Lopez-Rubio
Sep 19, 2014·Nature Protocols·Qiwei ShanCaixia Gao
Mar 6, 2015·Acta Biochimica Et Biophysica Sinica·He HuangYinhua Lu
Mar 26, 2015·ACS Synthetic Biology·Yaojun TongSang Yup Lee

❮ Previous
Next ❯

Citations

Dec 29, 2015·Metabolic Engineering·Tadas JakočiūnasJay D Keasling
Mar 6, 2016·Trends in Biotechnology·Ioannis MougiakosJohn van der Oost
Apr 9, 2016·Current Opinion in Biotechnology·Oksana Bilyk, Andriy Luzhetskyy
Sep 11, 2016·Journal of Industrial Microbiology & Biotechnology·Young Ji YooYeo Joon Yoon
Mar 10, 2017·Expert Opinion on Drug Discovery·Mingzi M ZhangHuimin Zhao
Apr 12, 2017·Nature Chemical Biology·Mingzi M ZhangHuimin Zhao
Apr 14, 2016·Natural Product Reports·Mingzi M ZhangHuimin Zhao
Jun 5, 2018·Biotechnology Journal·Yawei ZhaoYinhua Lu
Jun 20, 2018·Biotechnology Journal·Katia TarasavaRyan T Gill
Jul 20, 2018·Antibiotics·Ewa Maria Musiol-Kroll, Wolfgang Wohlleben
Jul 8, 2018·Applied and Environmental Microbiology·Lei LiYinhua Lu
Jun 5, 2016·Applied and Environmental Microbiology·Adam W WestbrookC Perry Chou
Dec 19, 2016·F1000Research·Keith F Chater
May 16, 2019·Biotechnology and Bioengineering·Wan Lin YeoFong Tian Wong
Jun 20, 2018·Journal of Industrial Microbiology & Biotechnology·Leonard KatzRichard H Baltz
Jan 27, 2019·Natural Product Reports·Fabrizio Alberti, Christophe Corre
Mar 17, 2019·Applied Microbiology and Biotechnology·Yu-Ting ZhangLu-Jing Ren
May 24, 2019·Applied Microbiology and Biotechnology·Jan KormanecLubomira Feckova
Dec 20, 2019·Applied Microbiology and Biotechnology·Qin LiuJing He
May 6, 2020·Journal of Industrial Microbiology & Biotechnology·Suhui YeEriko Takano
Jul 23, 2020·Frontiers in Bioengineering and Biotechnology·Jun ZhangYunzi Luo
Sep 16, 2017·Acta Biochimica Et Biophysica Sinica·Qi LiuDaijie Chen
Dec 15, 2018·Natural Product Reports·Yaojun TongSang Yup Lee
Sep 5, 2020·Microbial Cell Factories·Zhenquan LiuDawei Zhang
Dec 25, 2019·Science China. Life Sciences·Yawei ZhaoYinhua Lu
Dec 8, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Abhijit Dey
Jan 20, 2021·Angewandte Chemie·Mingming CaoBen Shen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biophysics of CRISPR

This feed focuses on broad characteristics of the CRISPR system and the proteins associated with it.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPRi Screens for Antibiotics

The CRISPR-Cas system is a gene editing technique that can be used for high-throughput genome-wide screens to identify modes of actions of novel antibiotics. Here is the latest research.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.