Highly efficient generation of definitive endoderm lineage from human induced pluripotent stem cells

Transplantation Proceedings
K SekineH Taniguchi

Abstract

Although hepatocytes can be an option for liver transplantation, the shortage of donor organs continues to worsen. Since the development of induced pluripotent stem (iPS) cell technology, it is eagerly anticipated to produce functional elements from pluripotent stem cells. These functional cells differentiated from iPS cells could be used for transplantation, drug screening, and in vitro toxicology. Human iPS cells are maintained on Mitomycin C-treated mouse embryonic fibroblast layers in DMEM-Ham F12-based medium supplemented with Knockout Serum Replacement, nonessential amino acids, 2-mercaptoethanol, and Glutamax. Differentiation of human iPS cells into a definitive endodermal lineage was induced with PRMI 1640 medium supplemented with B27 and 100 ng/mL human activin A. Two B27 supplements were examined with and without insulin. Furthermore, the PI3 kinase inhibitor LY294002 was used to examine the effect of inhibiting insulin signaling. We established efficient induction of definitive endodermal differentiation from iPS cells. Quantitative analysis revealed efficient (93.03 ± 2.74%) differentiation of human iPS cells into definitive endoderm cells using B27 minus insulin. This protocol may contribute as a fundamental techni...Continue Reading

References

Nov 1, 2005·Nature Biotechnology·Kevin A D'AmourEmmanuel E Baetge
Nov 29, 2005·Nature Biotechnology·Masahiro YasunagaShin-Ichi Nishikawa
Dec 10, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karim Si-TayebStephen A Duncan
May 13, 2011·Science Translational Medicine·Hua LiuYoon-Young Jang

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Citations

Sep 5, 2015·International Journal of Molecular Sciences·Chenxia Hu, Lanjuan Li
Nov 13, 2012·Trends in Molecular Medicine·Eyitayo S Fakunle, Jeanne F Loring
Aug 16, 2014·In Vitro Cellular & Developmental Biology. Animal·Hiromasa NinomiyaTatsuo Michiue
Nov 7, 2019·International Journal of Molecular Sciences·Daniel Rodríguez Gutiérrez, Anna Biason-Lauber
Nov 16, 2017·Current Protocols in Stem Cell Biology·Richard Siller, Gareth J Sullivan

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