Highly efficient nuclear delivery of anti-cancer drugs using a bio-functionalized reduced graphene oxide

Journal of Colloid and Interface Science
Xin Ting ZhengChang Ming Li

Abstract

Targeted drug delivery has become important, attractive and challenging in biomedical science and applications. Anti-HER2 antibody-conjugated poly-l-lysine functionalized reduced graphene oxide (anti-HER2-rGO-PLL) nanocarriers were prepared to efficiently deliver doxorubicin targeting at the nucleus of HER2 over-expressing cancer cells. The polycationic PLL was first covalently grafted to graphene oxide (GO) nanosheets followed by reduction to obtain rGO-PLL with high drug loading and good colloidal stability. The anti-HER2 antibodies were subsequently conjugated to the amino groups of PLL to achieve excellent cell uptake capability. Cellular uptake of anti-HER2-rGO-PLL into MCF7/HER2 cells is significantly higher than that of rGO-PLL due to the specific targeting of anti-HER2 to HER2 overexpressing breast cancer cells. Additionally the anti-HER2-rGO-PLL enables a fast accumulation of DOX inside the nucleus, its subcellular site of action. In vitro cytotoxicity measurements clearly reveal a seven fold improvement in the anticancer efficacy for anti-HER2-rGO-PLL/DOX in comparison to rGO-PLL/DOX. The enhanced anticancer efficacy could be ascribed to the different intracellular DOX distributions resulted from the different interna...Continue Reading

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Citations

May 25, 2016·Journal of Colloid and Interface Science·Lian Ying ZhangChang Ming Li
Nov 5, 2016·International Journal of Biological Macromolecules·Na MengYinghong Xiao
Sep 3, 2016·International Journal of Molecular Sciences·Panoraia I SiafakaDimitrios N Bikiaris
Sep 21, 2016·Journal of Drug Targeting·Nazanin RahmanianYadollah Omidi
Dec 10, 2016·Journal of Biomaterials Science. Polymer Edition·Junmei WangSufang Li
Apr 13, 2017·Biomaterials Science·Shine AugustineBansi D Malhotra
May 12, 2017·International Journal of Molecular Sciences·Musafar Gani SikkandharParasuraman Padmanabhan
Aug 5, 2017·Advanced Healthcare Materials·Nagappa L Teradal, Raz Jelinek
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Jun 29, 2021·Frontiers in Bioengineering and Biotechnology·Julia DriscollTushar Patel
Jul 17, 2021·Journal of Nanobiotechnology·Guangman CuiGuangyu Yao
Oct 11, 2017·ACS Applied Materials & Interfaces·Qianshun LiYunfeng Lin
Nov 15, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Ali PourjavadiMehdi Akhlaghi
Jan 19, 2021·ACS Biomaterials Science & Engineering·Zahra Mohammadpour, Keivan Majidzadeh-A

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