Highly efficient nuclear DNA typing of the World War II skeletal remains using three new autosomal short tandem repeat amplification kits with the extended European Standard Set of loci.

Croatian Medical Journal
Irena Zupanic PajnicBorut Stefanic

Abstract

To perform an efficiency study of three new amplification kits with the extended European Standard Set (ESS) of loci for autosomal short tandem repeat (STR) typing of skeletal remains excavated from the World War II mass graves in Slovenia. In the beginning of the 2011, we analyzed 102 bones and teeth using the PowerPlex ESX 17 System (Promega), AmpFiSTR NGM PCR Amplification Kit (Applied Biosystems), and Investigator ESSplex Kit (Qiagen). We cleaned the bones and teeth, removed surface contamination, and ground them into a powder using liquid nitrogen. Prior to DNA isolation with Biorobot EZ1 (Qiagen), 0.5 g bone or tooth powder was decalcified. Nuclear DNA of the samples was quantified using real-time polymerase chain reaction. All three kits used the same extract with the amplification conditions recommended by the manufacturers. We extracted up to 131 ng DNA/g of powder from the bones and teeth. All three amplification kits showed very similar efficiency, since DNA typing was successful with all amplification kits in 101 out of 102 bones and teeth, which represents a 99% success rate. The commercially available ESX 17, ESSplex, and NGM kits are highly reliable for STR typing of World War II skeletal remains with the DNA ext...Continue Reading

References

Oct 22, 1998·European Journal of Human Genetics : EJHG·E JehaesJ J Cassiman
Jan 5, 2000·American Journal of Physical Anthropology·C J Kolman, N Tuross
Apr 12, 2001·International Journal of Legal Medicine·K AnslingerW Eisenmenger
Oct 29, 2003·Journal of Human Evolution·Colin I SmithMatthew J Collins
Jul 9, 2005·Forensic Science International : Synergy·Peter GillPeter M Schneider
Sep 27, 2005·Forensic Science International : Synergy·Brian M Kemp, David Glenn Smith
Jan 21, 2006·Forensic Science International : Synergy·Peter GillPeter M Schneider
Feb 24, 2007·International Journal of Legal Medicine·Kirsty ShawBarbara Daniel
Jul 25, 2007·Journal of Forensic Sciences·Jodi A IrwinMark D Leney
Oct 20, 2007·Journal of Forensic Sciences·Jodi A IrwinThomas J Parsons
Dec 17, 2008·Forensic Science International. Genetics·Cecilia AnderungAnders Götherström
Apr 17, 2009·Journal of Forensic Sciences·Lisa M MisnerDavid R Foran
Jul 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wiesław BogdanowiczTomasz Kupiec
Mar 11, 2010·International Journal of Legal Medicine·Irena Zupanic PajnicJoze Balazic
Apr 14, 2010·Journal of Forensic Sciences·Esther J LeeDawnie Wolfe Steadman
May 12, 2010·Journal of Forensic Sciences·Hwan Young LeeKyoung-Jin Shin
May 12, 2010·Forensic Science International. Genetics·Hwan Young LeeKyoung-Jin Shin
Jul 8, 2010·Forensic Science International. Genetics·Aliz MolnárHorolma Pamjav
Jul 14, 2010·Legal Medicine·Agnieszka Parys-ProszekWojciech Branicki
Sep 30, 2010·International Journal of Legal Medicine·Bruce BudowleLori Hennessy
Nov 13, 2010·Forensic Science International. Genetics·Valerie C TuckerDouglas R Storts

❮ Previous
Next ❯

Citations

Oct 27, 2012·Croatian Medical Journal·Melanie HarderNicole von Wurmb-Schwark
Nov 30, 2015·Journal of Forensic and Legal Medicine·Irena Zupanič PajničKsenija Geršak
Oct 9, 2015·Forensic Science International. Genetics·Jacek DrabikWitold Pepinski
Mar 27, 2015·Journal of Forensic Sciences·Inge MorildThomas J Parsons
Aug 21, 2013·Forensic Science International. Genetics·Mariela CaputoDaniel Corach
Sep 20, 2015·Forensic Science International : Synergy·Esther Alia-GarcíaRosa Del Campo
Nov 5, 2016·Journal of Forensic and Legal Medicine·Irena Zupanič PajničKsenija Geršak
May 20, 2017·Journal of Forensic Sciences·Emely Morales ColónTracey Dawson Cruz
Dec 23, 2016·Electrophoresis·William Goodwin, Sharizah Alimat
Mar 28, 2019·Forensic Sciences Research·Krista E Latham, Jessica J Miller
Nov 29, 2020·Forensic Science International. Genetics·Tomaž ZupancIrena Zupanič Pajnič
Jun 8, 2021·Forensic Science International : Synergy·Caterina RaffoneMarian M de Pancorbo
Jun 11, 2021·Scientific Reports·Diego Lozano-PeralStella Martín-de-Las-Heras
Jun 27, 2021·Science & Justice : Journal of the Forensic Science Society·Meghan MckinnonDenice Higgins

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

ESSplex
GeneMapper ID
NGM
SDS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.