PMID: 7535763Dec 1, 1994Paper

Highly efficient transfection into primary cultured mouse hepatocytes by use of cation-liposomes: an application for immunization

Journal of Biochemistry
Y WatanabeT Akaike

Abstract

Transfection methods for primary cultured mouse hepatocytes were examined. Of four conventional transfection methods examined, involving use of DEAE-dextran, calcium phosphate, cation-liposomes (lipofection), and cation-multilamellar liposomes, only cation-liposomes induced highly efficient transfection into primary cultured mouse hepatocytes. The highest transfection rate reached more than 60% of the total cells. Three other commonly used cell types (CHO-K1, COS-1, 3T3-L1) were also tested as target cells, but highly efficient transfection was observed specifically in primary cultured mouse hepatocytes. The transfection remained at a high level from 6 to 48 h after the start of incubation with the cation-liposome-DNA complex in the absence of serum, and the transfection rate decreased in inverse relation to the increase in cell density. The transfection was inhibited by free low density lipoprotein (LDL), EDTA, and an endocytosis inhibitor, cytochalasin B. These data suggest that the transfection is mediated not only by membrane fusion, as is generally accepted, but also by endocytosis. This information should be useful for research in hepatocyte biology and the development of gene therapy. As one of the applications, simple a...Continue Reading

Citations

Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·I van der WoudeD Hoekstra
Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I AjiokaY Watanabe
Jun 1, 2000·Nature·L BildiriciD Rickwood
Nov 30, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yoshiko UchiyamaTsuguo Sawada
Jan 1, 1997·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·B WollenbergR Zeidler
Mar 22, 2001·Transplantation·P H TanA J George
Mar 16, 2000·In Vitro Cellular & Developmental Biology. Animal·C A HollmannJ S Butel
Jul 7, 1997·Advanced Drug Delivery Reviews·R NivenJ Smith

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