Highly Flexible Platform for Tuning Surface Properties of Silica Nanoparticles and Monitoring Their Biological Interaction

ACS Applied Materials & Interfaces
Isaac Ojea-JiménezDouglas Gilliland

Abstract

The following work presents a simple, reliable and scalable seeding-growth methodology to prepare silica nanoparticles (SiO2 NPs) (20, 30, 50 and 80 nm) directly in aqueous phase, both as plain- as well as fluorescent-labeled silica. The amount of fluorescent label per particle remained constant regardless of size, which facilitates measurements in terms of number-based concentrations. SiO2 NPs in dispersion were functionalized with an epoxysilane, thus providing a flexible platform for the covalent linkage of wide variety of molecules under mild experimental conditions. This approach was validated with ethylenediamine, two different amino acids and three akylamines to generate a variety of surface modifications. Accurate characterization of particle size, size distributions, morphology and surface chemistry is provided, both for as-synthesized particles and after incubation in cell culture medium. The impact of physicochemical properties of SiO2 NPs was investigated with human alveolar basal epithelial cells (A549) such as the effect in cytotoxicity, cell internalization and membrane interaction.

References

Mar 1, 1992·British Journal of Industrial Medicine·N Bagchi
Aug 12, 2003·Journal of Nanoscience and Nanotechnology·Rovelyn TapecWeihong Tan
Oct 9, 2004·International Journal of Hygiene and Environmental Health·Ralf WottrichHarald F Krug
Jul 16, 2005·Journal of Colloid and Interface Science·Yangyang SunC P Wong
Jan 7, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Paul BormStewart Wood
Feb 4, 2006·Science·Andre NelNing Li
Oct 19, 2006·Journal of the American Chemical Society·Toshiyuki YokoiTakashi Tatsumi
Nov 23, 2006·Toxicology and Applied Pharmacology·Weisheng LinYinfa Ma
Mar 27, 2007·Journal of Colloid and Interface Science·Yanqing AnWeimin Liu
Jul 17, 2007·Chemical Research in Toxicology·Yuhui JinJulia Xiaojun Zhao
Sep 18, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Richard C MurdockSaber M Hussain
Jan 30, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Kurtis D HartlenVladimir Kitaev
Apr 11, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dominique LisonJohan A Martens
May 27, 2008·Journal of Colloid and Interface Science·Oliver WeicholdMartin Möller
Jun 3, 2008·Advanced Drug Delivery Reviews·Igor I SlowingVictor S-Y Lin
Sep 30, 2008·Journal of Colloid and Interface Science·P VejayakumaranC K Chee
Feb 3, 2009·Journal of Inorganic Biochemistry·Soo-Jin ChoiJin-Ho Choy
Aug 25, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Leen C J ThomassenJohan A Martens
Oct 24, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jorina GeysPeter H M Hoet
Apr 15, 2010·Journal of Toxicology and Environmental Health. Part a·Yongli ShiHe Wang
Dec 4, 2010·Particle and Fibre Toxicology·Dorota NapierskaPeter H Hoet
Apr 10, 2012·Environmental Science & Technology·Tamara DiedrichEric H Oelkers
Oct 17, 2012·Journal of Colloid and Interface Science·Marcell PálmaiAttila Bóta
Nov 5, 2014·Surface Science Reports·Alexander LibermanAndrew C Kummel
Jul 15, 2015·Regulatory Toxicology and Pharmacology : RTP·Valeria AmentaRuud J Peters
Oct 31, 2015·PloS One·Blanka Halamoda-KenzaouiAgnieszka Kinsner-Ovaskainen

❮ Previous
Next ❯

Citations

Mar 24, 2018·Methods and Applications in Fluorescence·Ying LianXu-Dong Wang
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Hainan SunShumei Zhai
Nov 9, 2019·Chemical Research in Toxicology·Todd A StueckleCerasela Z Dinu
Aug 12, 2017·ACS Applied Materials & Interfaces·Alixandra WagnerCerasela Zoica Dinu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.