Highly multiparametric analysis by mass cytometry

Journal of Immunological Methods
Olga OrnatskyScott Tanner

Abstract

This review paper describes a new technology, mass cytometry, that addresses applications typically run by flow cytometer analyzers, but extends the capability to highly multiparametric analysis. The detection technology is based on atomic mass spectrometry. It offers quantitation, specificity and dynamic range of mass spectrometry in a format that is familiar to flow cytometry practitioners. The mass cytometer does not require compensation, allowing the application of statistical techniques; this has been impossible given the constraints of fluorescence noise with traditional cytometry instruments. Instead of "colors" the mass cytometer "reads" the stable isotope tags attached to antibodies using metal-chelating labeling reagents. Because there are many available stable isotopes, and the mass spectrometer provides exquisite resolution between detection channels, many parameters can be measured as easily as one. For example, in a single tube the technique allows for the ready detection and characterization of the major cell subsets in blood or bone marrow. Here we describe mass cytometric immunophenotyping of human leukemia cell lines and leukemia patient samples, differential cell analysis of normal peripheral and umbilical co...Continue Reading

References

Feb 2, 1999·Journal of the American Society for Mass Spectrometry·I I Stewart, J W Olesik
Jul 18, 2002·Nature Reviews. Drug Discovery·Kevin BraeckmansJoseph Demeester
Aug 3, 2004·Nature Reviews. Immunology·Stephen P PerfettoMario Roederer
Dec 13, 2005·Journal of Immunological Methods·O OrnatskyJ Dick
Feb 17, 2006·Methods : a Companion to Methods in Enzymology·Mohamed F Elshal, J Philip McCoy
Apr 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·John P Nolan, Francis Mandy
Jul 1, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Brian Z RingDouglas T Ross
Jul 25, 2006·Nature Medicine·Pratip K ChattopadhyayMario Roederer
Aug 23, 2006·Analytical and Bioanalytical Chemistry·J BettmerA Prange
Jan 17, 2007·Analytical and Bioanalytical Chemistry·Maria CareriClaudio Mucchino
Jan 18, 2007·Cell Proliferation·M J BartenJ F Gummert
May 30, 2007·Angewandte Chemie·Xudong LouMitchell A Winnik
Aug 25, 2007·Theoretical Biology & Medical Modelling·Brian P HanleyR Holland Cheng
Mar 29, 2008·Cell Stem Cell·Ravindra MajetiIrving L Weissman
Apr 3, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bridget E McLaughlinDavid M Asmuth
Jul 17, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Carlos E PedreiraUNKNOWN EuroFlow Consortium
Oct 24, 2008·Analytical and Bioanalytical Chemistry·Maria CareriAlessandro Mangia
Jan 6, 2009·Journal of Analytical Atomic Spectrometry·Olga I OrnatskyMitchell A Winnik
Jan 13, 2009·Immunology·Pratip K ChattopadhyayMario Roederer
Sep 29, 2009·Molecular & Cellular Proteomics : MCP·José M A MoreiraIrina Gromova
Jan 26, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Patrick AutissierKenneth C Williams
Apr 15, 2010·Journal of Analytical Atomic Spectrometry·Ahmed I AbdelrahmanMitchell A Winnik

❮ Previous
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Citations

Oct 20, 2012·Analytical and Bioanalytical Chemistry·Stefan Vogt, Martina Ralle
Apr 9, 2013·Cancer Immunology, Immunotherapy : CII·Scott D TannerThaddeus C George
Feb 22, 2012·Nature Reviews. Immunology·Holden T MaeckerRobert Nussenblatt
Jun 1, 2012·Nature Reviews. Rheumatology·Holden T MaeckerC Garrison Fathman
Jan 15, 2013·Current Opinion in HIV and AIDS·Matthew C Strain, Douglas D Richman
Nov 16, 2012·PloS One·Paula FernandezUNKNOWN Swiss Cytometry Society
Jan 15, 2014·Nature Methods·Christoph ZechnerHeinz Koeppl
Feb 4, 2014·Cancer Immunology, Immunotherapy : CII·Serena ChangHolden T Maecker
Dec 3, 2013·Analytical and Bioanalytical Chemistry·Thomas HaselgrüblerJan Hesse
Oct 2, 2014·Nature Cell Biology·Philipp S HoppeTimm Schroeder
Sep 25, 2014·Immunology Letters·José-Enrique O'ConnorRobert C Callaghan
Sep 25, 2014·Immunology Letters·José-Enrique O'ConnorRobert C Callaghan
Mar 7, 2012·Cellular and Molecular Life Sciences : CMLS·Krisztián PappJózsef Prechl
Nov 10, 2012·Critical Reviews in Clinical Laboratory Sciences·Zbigniew DarzynkiewiczDonald Wlodkowic
Dec 22, 2012·Expert Review of Proteomics·Ferdinando MannelloMauro Magnani
Apr 6, 2013·Expert Review of Clinical Immunology·Petter BrodinMarkus J Maeurer
Jun 20, 2012·Expert Opinion on Drug Discovery·J Paul RobinsonVincent Jo Davisson
May 24, 2013·Immune Network·Erika A O'DonnellRavi Hingorani
Dec 15, 2012·Seminars in Immunology·Rachel A GottschalkRonald N Germain
Dec 19, 2012·Current Opinion in Biotechnology·Stanislav S RubakhinJonathan V Sweedler
May 18, 2012·Trends in Parasitology·Tuan M TranPeter D Crompton
Apr 17, 2012·Methods : a Companion to Methods in Enzymology·Qing Chang, David Hedley
Aug 16, 2014·Current Opinion in Biotechnology·Serena Di Palma, Bernd Bodenmiller
Dec 2, 2015·Immunology and Cell Biology·Giacomo Donati
May 10, 2015·Journal of Immunological Methods·David Martino, Katrina Allen
Jul 22, 2014·International Journal of Cancer. Journal International Du Cancer·Qing ChangDavid W Hedley
Apr 24, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michael D Leipold
Mar 21, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rachel FinckSean C Bendall
Jul 21, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·László ImreGábor Szabó
May 12, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Harris G FienbergBernd Bodenmiller
Jun 14, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Zbigniew Darzynkiewicz
Jul 31, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Andrea Cossarizza, Andreas Radbruch
Sep 12, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Henrik E MeiHolden T Maecker
Oct 10, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Nima AghaeepourRyan R Brinkman
Apr 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Lili WangRichard Stebbings
Jun 7, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·Martin A Jünger, Ruedi Aebersold
Feb 18, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Tim R MosmannGaurav Sharma
Dec 20, 2014·Proteomics. Clinical Applications·Priscila Camillo TeixeiraPaul Cutler
Jan 28, 2015·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Naeha SubramanianBhaskar Dutta

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