Highly parallel remote SPR detection of DNA hybridization by micropillar optical arrays

Analytical and Bioanalytical Chemistry
Karim VindasElodie Engel

Abstract

Remote detection by surface plasmon resonance (SPR) is demonstrated through microstructured optical arrays of conical nanotips or micropillars. Both geometries were fabricated by controlled wet chemical etching of bundles comprising several thousands of individual optical fibers. Their surface was coated by a thin gold layer in order to confer SPR properties. The sensitivity and resolution of both shapes were evaluated as a function of global optical index changes in remote detection mode performed by imaging through the etched optical fiber bundle itself. With optimized geometry of micropillar arrays, resolution was increased up to 10-4 refractive index units. The gold-coated micropillar arrays were functionalized with DNA and were able to monitor remotely the kinetics of DNA hybridization with complementary strands. We demonstrate for the first time highly parallel remote SPR detection of DNA via microstructured optical arrays. The obtained SPR sensitivity combined with the remote intrinsic properties of the optical fiber bundles should find promising applications in biosensing, remote SPR imaging, a lab-on-fiber platform dedicated to biomolecular analysis, and in vivo endoscopic diagnosis. Graphical abstract We present a sin...Continue Reading

References

Jun 1, 2001·Physics in Medicine and Biology·A FensterH N Cardinal
Apr 20, 2006·Biosensors & Bioelectronics·Ilaria MannelliMichael Canva
Sep 29, 2006·Analytical and Bioanalytical Chemistry·Yong-Jin LiZhi-Ping Zhang
Nov 23, 2006·Nature Medicine·Seok H YunBrett E Bouma
Oct 17, 2007·The FEBS Journal·Hans H GorrisDavid R Walt
Jan 31, 2008·Chemical Reviews·Christopher N LaFratta, David R Walt
Sep 25, 2009·Biosensors & Bioelectronics·Jeroen PolletJeroen Lammertyn
Nov 17, 2009·Analytical and Bioanalytical Chemistry·F DeissP R Stoddart
Apr 8, 2010·Optics Express·Heykel AouaniHervé Rigneault
Jul 3, 2013·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Venkataraman Subramanian, Krish Ragunath
Feb 12, 2014·Applied Optics·Jennifer S HartleyPaul R Stoddart
Jan 27, 2015·Analytical and Bioanalytical Chemistry·Christophe CaucheteurJacques Albert
Sep 20, 2012·The Journal of Physical Chemistry Letters·Giuseppe Spoto, Maria Minunni
Apr 19, 2016·Journal of Biophotonics·Ayda ArayFrancesco Baldini
Apr 5, 2017·Nanotechnology·Iulia AntoheJeroen Lammertyn
Nov 18, 2017·Clinical Neurology and Neurosurgery·Helen K B FuzariDaniella A de Oliveira
Apr 14, 2018·Analytical and Bioanalytical Chemistry·Yu-Wen Su, Wei Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.