Highly pathogenic porcine reproductive and respiratory syndrome virus induces prostaglandin E2 production through cyclooxygenase 1, which is dependent on the ERK1/2-p-C/EBP-β pathway

Journal of Virology
Yanmin BiWen-hai Feng

Abstract

Atypical porcine reproductive and respiratory syndrome (PRRS) caused by highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is characterized by high fever and high mortality. However, the mechanism underlying the fever induction is still unknown. Prostaglandin E2 (PGE2), synthesized by cyclooxygenase type 1/2 (COX-1/2) enzymes, is essential for inducing fever. In this study, we found that PGE2, together with COX-1, was significantly elevated by HP-PRRSV. We subsequently demonstrated that extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK (p-ERK) were the key nodes to trigger COX-1 expression after HP-PRRSV infection. Furthermore, we proved the direct binding of p-C/EBP-β to the COX-1 promoter by luciferase reporter and chromatin immunoprecipitation assays. In addition, silencing of C/EBP-β remarkably impaired the enhancement of COX-1 production induced by HP-PRRSV infection. Taken together, our results indicate that HP-PPRSV elicits the expression of COX-1 through the ERK1/2-p-C/EBP-β signaling pathway, resulting in the increase of PGE2, which might be the cause of high fever in infected pigs. Our findings might provide new insights into the molecular mechanisms underlying the...Continue Reading

References

Jan 1, 1990·Cancer Immunology, Immunotherapy : CII·S Rasoul-RockenschaubC Kunz
Jun 1, 1988·Journal of Applied Physiology·K L BrighamJ A Oates
May 1, 1982·The American Journal of Medicine·C A Dinarello, S M Wolff
Apr 1, 1996·The American Journal of Physiology·A GreshamA P Pentland
Mar 1, 1997·American Journal of Respiratory and Critical Care Medicine·T ViganòG Folco
Apr 16, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·D W GilroyD A Willoughby
Apr 23, 2002·Alimentary Pharmacology & Therapeutics·A TanakaK Takeuchi
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Lyne I LemieuxChris R J Kennedy
Apr 1, 2003·Advances in Experimental Medicine and Biology·Peter DieterAngelika Kolada
Aug 28, 2003·Journal of Cellular Physiology·Jian-Xiong ChenBarbara Meyrick
Sep 6, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K MizumuraT Horie
Dec 31, 2003·Trends in Immunology·Carlo VancheriNunzio Crimi
Sep 4, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chikao ShimamotoTakashi Nakahari
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Takakazu Oka
Oct 28, 2004·Veterinary Immunology and Immunopathology·Udeni B R Balasuriya, N James MacLachlan
Jun 22, 2005·Prostaglandins & Other Lipid Mediators·Clark M BlatteisVit Perlik
Jul 28, 2005·The Journal of Steroid Biochemistry and Molecular Biology·George P StudzinskiJonathan S Harrison
Aug 29, 2006·PLoS Biology·Alexandre A SteinerAndrej A Romanovsky
Dec 13, 2006·Respiratory Physiology & Neurobiology·Guangfan Zhang, Lu-Yuan Lee
Sep 7, 2007·Annals of the New York Academy of Sciences·L Guilherme, J Kalil
Jul 29, 2008·Journal of Cellular and Molecular Medicine·Nina ZidarDusan Stajer
Oct 4, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kelly A Brant, James P Fabisiak
Dec 31, 2008·Prostaglandins & Other Lipid Mediators·Duanpen SandeeKanyawim Kirtikara
Feb 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiang-Dong LiuShu-Hong Zhao
Feb 16, 2013·Food and Environmental Virology·YingShun ZhouHaoCheng Li

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Citations

Dec 13, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xuegang LuoGaiping Zhang

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