Highly photostable wide-dynamic-range pH sensitive semiconducting polymer dots enabled by dendronizing the near-IR emitters

Chemical Science
Lei ChenD T Chiu

Abstract

One constraint of semiconducting polymer dots (Pdots), especially those with near-IR emission, is their low effective emitter ratio (∼1.5 mole percent), which limits their pH sensing performance. The other critical issue of existing Pdot-based pH sensors is their poor photostability. To address these issues, we developed a series of Pdots by dendronizing the squaraine-based pH responsive near-IR emitter, which is covalently incorporated into the polyfluorene (PFO) backbone. The fluorescence self-quenching of the NIR squaraine emitter was effectively suppressed at a high emitter concentration of 5 mole percent. Through controlling the individually incomplete energy transfer from the amorphous PFO donor to the blue β-phase PFO and NIR squaraine emitter, we obtained a ratiometric pH sensor with simultaneously improved pH sensitivity, brightness, and photostability. The Pdots showed a fast and reversible pH response over the whole biological pH range of 4.7 to 8.5. Intracellular pH mapping was successfully demonstrated using this ultra-bright and photostable Pdot-based pH indicator.

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Citations

Mar 24, 2018·Chemical Society Reviews·Xiaolin HuangXiaoyuan Chen
Oct 23, 2018·Chembiochem : a European Journal of Chemical Biology·Xiaofeng FangChangfeng Wu
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bing ChenFeng Wang
Aug 12, 2020·Biomaterials Science·Ye YuanChangfeng Wu
Jul 21, 2021·Chemical Reviews·W Russ AlgarHyungki Kim
Oct 3, 2018·Analytical Chemistry·Chun-Ting KuoDaniel T Chiu
Dec 9, 2021·Biomedical Materials·Haoying HuangChunyan Li

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Methods Mentioned

BETA
nanoprecipitation
dynamic light scattering
fluorescence imaging

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