Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration

Macromolecular Bioscience
Sofia AmadoriAdriana Bigi

Abstract

3D highly porous (93% total porosity) gelatin scaffolds were prepared according to a novel, simple method, which implies gelatin foaming, gelification, soaking into ethanol and successive freeze-drying. Reinforcement of the as-prepared scaffolds (GEL) was performed through immersion in aqueous solutions at different gelatin concentrations. Reinforcement solutions with and without genipin addition allowed to prepare two series of samples:cross-linked and uncross-linked samples, respectively. The amount of gelatin adsorbed onto the reinforced samples increases as a function of gelatin concentration in solution and provokes a drastic improvement of the compressive modulus and collapse strength up to values of about 30 and 4 MPa, respectively. The open and interconnected porosity, although slightly reduced, is still of the order of 80% in the samples reinforced with the highest concentration of gelatin. Water uptake ability evaluated after immersion in PBS for 20 s decreases with gelatin reinforcement. The presence of genipin in cross-linked samples reduces gelatin release and stabilizes the scaffolds in solution. Chondrocytes from human articular cartilage adhere, proliferate, and penetrate into the scaffolds. The evaluation of di...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Nov 19, 2005·Science·Molly M Stevens, Julian H George
Mar 14, 2007·Acta Biomaterialia·Brendan A HarleyLorna J Gibson
Jul 4, 2007·Journal of Biomedical Materials Research. Part a·Guo-Chung DongChun-Hsu Yao
Oct 18, 2007·Biopolymers·Julie Glowacki, Shuichi Mizuno
May 22, 2009·Nature Materials·Elsie S PlaceMolly M Stevens
Jul 20, 2011·Journal of Polymer Science. Part B, Polymer Physics·Bret D UleryCato T Laurencin
May 23, 2012·Journal of Biomedical Materials Research. Part a·Piergiorgio GentileGianluca Ciardelli
May 1, 2013·Journal of Biomedical Materials Research. Part a·Silvia PanzavoltaAdriana Bigi
Jan 5, 2014·Acta Biomaterialia·Qin ZhangGuoping Chen
Jan 15, 2014·Tissue Engineering. Part B, Reviews·Kazunori ShimomuraNorimasa Nakamura
Jan 15, 2014·Acta Biomaterialia·Tanya J LevingstoneJohn P Gleeson
Jan 1, 2013·Materials Science & Engineering. C, Materials for Biological Applications·C JelenG Vozzi
Jan 13, 2015·Materials Science & Engineering. C, Materials for Biological Applications·S Ali PoursamarA P M Antunes
Jan 30, 2015·Journal of Materials Science. Materials in Medicine·Sofia AmadoriAdriana Bigi

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Citations

Mar 10, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Shane D Frazier, Wil V Srubar
Mar 25, 2016·Current Opinion in Biotechnology·Nancy LeeHelen Lu
Aug 5, 2017·International Journal of Molecular Sciences·Jung Min LeeChan Hum Park
Jun 8, 2018·Macromolecular Bioscience·Silvia PanzavoltaAdriana Bigi
Jun 1, 2018·BioMed Research International·Yong HeDaping Wang
May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Francesca Di FilippoSilvia Panzavolta
May 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shane D FrazierWil V Srubar
Oct 18, 2016·Macromolecular Bioscience·Silvia PanzavoltaAdriana Bigi
Nov 19, 2020·Polymers·Mior Muhammad Amirul ArifMohd Heikal Mohd Yunus

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