Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2

Stem Cells International
Xiangfen LiDingming Huang

Abstract

Human dental pulp cells (HDPCs) play a vital role in dentin formation and reparative dentinogenesis, which indicated their potential application in regenerative medicine. However, HDPCs, which can only be obtained from scarce human pulp tissues, also have a limited lifespan in vitro, and stem cells usually lose their original characteristics over a large number of passages. To overcome these challenges, we successfully immortalized human dental pulp cells using the piggyBac system which was employed to efficiently overexpress the SV40 T-Ag, and we then comprehensively described the cell biological behavior. The immortalized human dental pulp cells (iHDPCs) acquired long-term proliferative activity and expressed most HDPC markers. The iHDPCs maintained multiple differentiation potential and could be induced to differentiate into chondrogenic, osteogenic, and adipogenic cells in vitro. We also proved that the iHDPCs gained a stronger ability to migrate than the primary cells, while apoptosis was inhibited. Furthermore, highly proliferative iHDPCs displayed no oncogenicity when subcutaneously implanted into athymic nude mice. Finally, iHDPCs exhibited odontogenic differentiation ability and secreted dentin sialophosphoprotein (DSP...Continue Reading

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Citations

Oct 10, 2020·World Journal of Stem Cells·Cornelia Larissa Granz, Ali Gorji
May 1, 2021·International Journal of Environmental Research and Public Health·Julia Guerrero-GironésMiguel R Pecci-Lloret
Oct 16, 2021·Frontiers in Cell and Developmental Biology·Xiangfen LiJake Chen

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Methods Mentioned

BETA
electrophoresis
PCR
flow cytometry

Software Mentioned

InCyte

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