Highly Promiscuous Oxidases Discovered in the Bovine Rumen Microbiome

Frontiers in Microbiology
Lisa UfartéElisabeth Laville

Abstract

The bovine rumen hosts a diverse microbiota, which is highly specialized in the degradation of lignocellulose. Ruminal bacteria, in particular, are well equipped to deconstruct plant cell wall polysaccharides. Nevertheless, their potential role in the breakdown of the lignin network has never been investigated. In this study, we used functional metagenomics to identify bacterial redox enzymes acting on polyaromatic compounds. A new methodology was developed to explore the potential of uncultured microbes to degrade lignin derivatives, namely kraft lignin and lignosulfonate. From a fosmid library covering 0.7 Gb of metagenomic DNA, three hit clones were identified, producing enzymes able to oxidize a wide variety of polyaromatic compounds without the need for the addition of copper, manganese, or mediators. These promiscuous redox enzymes could thus be of potential interest both in plant biomass refining and dye remediation. The enzymes were derived from uncultured Clostridia, and belong to complex gene clusters involving proteins of different functional types, including hemicellulases, which likely work in synergy to produce substrate degradation.

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Citations

May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jéssica P SilvaEliane F Noronha

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Datasets Mentioned

BETA
LT674549
LT674550

Methods Mentioned

BETA
ABTS
chemical treatment

Software Mentioned

BLASTX
MEGAN
InterProscan
BLAST
Crossmatch
RPS
MetaGene
BLASTP

Related Concepts