PMID: 8943037Nov 26, 1996Paper

Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning

Proceedings of the National Academy of Sciences of the United States of America
V LarionovM A Resnick

Abstract

Transformation-associated recombination (TAR) can be exploited in yeast to clone human DNAs. TAR cloning was previously accomplished using one or two telomere-containing vectors with a common human repeat(s) that could recombine with human DNA during transformation to generate yeast artificial chromosomes (YACs). On basis of the proposal that broken DNA ends are more recombinogenic than internal sequences, we have investigated if TAR cloning could be applied to the generation of circular YACs by using a single centromere vector containing various human repeats at opposite ends. Transformation with these vectors along with human DNA led to the efficient isolation of circular YACs with a mean size of approximately 150 kb. The circular YACs are stable and they can be easily separated from yeast chromosomes or moved into bacterial cells if the TAR vector contains an Escherichia coli F-factor cassette. More importantly, circular TAR cloning enabled the selective isolation of human DNAs from monochromosomal human-rodent hybrid cell lines. Although < 2% of the DNA in the hybrid cells was human, as much as 80% of transformants had human DNA YACs when a TAR cloning vector contained Alu repeats. The level of enrichment of human DNA was n...Continue Reading

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Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·V LarionovM A Resnick

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Citations

Feb 11, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M R CancillaN Y Kouprina
Oct 23, 1997·Gene·N KouprinaV Larionov
Jul 1, 1997·Genetic Analysis : Biomolecular Engineering·E FrengenP J de Jong
Aug 23, 2000·Nucleic Acids Research·M CocchiaR Nagaraja
Mar 10, 2010·Nucleic Acids Research·Gwynedd A BendersClyde A Hutchison
Mar 22, 2008·Genome Génome / Conseil National De Recherches Canada·S-H LeemV Larionov
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N KouprinaV Larionov
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·V LarionovM A Resnick
Dec 21, 2002·Immunological Reviews·Daniel E GeraghtyAkiko Ishitani
Nov 26, 2003·FEMS Microbiology Reviews·Natalay Kouprina, Vladimir Larionov
Jan 25, 2017·ACS Synthetic Biology·Fabien RideauCécile Bébéar
Apr 23, 2008·Current Protocols in Human Genetics·Natalay Kouprina, Vladimir Larionov
Aug 4, 2018·ACS Synthetic Biology·Natalay KouprinaVladimir Larionov

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