Highly Selective Mitochondrial Targeting by a Ruthenium(II) Peptide Conjugate: Imaging and Photoinduced Damage of Mitochondrial DNA

Angewandte Chemie
Christopher S BurkeTia E Keyes

Abstract

Mitochondrial DNA (mtDNA) plays a crucial but incompletely understood role in cellular biochemistry and etiology of numerous disease states. Thus, there is an urgent need for targeted probes that can dynamically respond to changes to mtDNA such as copy number in live cells, but it is difficult to permeate the mitochondrial membrane of the living cell. Now, a ruthenium(II) light-switching probe targeted by peptide vectorization selectively to mitochondrial nucleoids is presented. Evidence for DNA binding by the probe in live cells is derived from confocal fluorescence microscopy, resonance Raman, and luminescence lifetime imaging. While viable under imaging conditions, specific staining of mitochondrial DNA permitted efficient and selective photoinduced toxicity on a cell-by-cell basis under higher excitation intensities. This powerful combination of imaging and photocytotoxicity is an important step towards realizing phototheranostic application of such RuII probes.

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Citations

Sep 13, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Karmel Sofia GkikaTia E Keyes
Apr 22, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·João Franco MachadoTânia S Morais
Jul 17, 2020·Chemical Communications : Chem Comm·Sandra Estalayo-AdriánThorfinnur Gunnlaugsson
Sep 15, 2020·Macromolecular Bioscience·Nobuyuki MorimotoMasaya Yamamoto
Feb 9, 2021·Organic & Biomolecular Chemistry·Tarushyam MukherjeeSriram Kanvah
Oct 17, 2020·The Journal of Biological Chemistry·David L ProleNick S Jones
Feb 18, 2021·Journal of Inorganic Biochemistry·Federica GuarraTarita Biver
Jan 18, 2021·The Journal of Biological Chemistry·David L ProleNick S Jones
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·João Franco MachadoTânia S Morais
Jul 23, 2021·Journal of the American Chemical Society·Wen-Jin WangZong-Wan Mao
Mar 30, 2019·Journal of the American Chemical Society·Chaolong LiuJingli Yuan
Dec 12, 2018·Journal of the American Chemical Society·Lucien N LameijerSylvestre Bonnet
Sep 23, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Daniel ObitzNils Metzler-Nolte

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