Highly Selective, Naked-Eye, and Trace Discrimination between Perfect-Match and Mismatch Sequences Using a Plasmonic Nanoplatform

Analytical Chemistry
Cuiyun YinCuisong Zhou

Abstract

A plasmonic nanoplatform to perform an enzyme-free, naked-eye, and trace discrimination of single-base mutation from fully matched sequence is reported. The nanoplatform showed great potential to enhance catalytic hairpin assembly (CHA) amplification efficiency and biocatalytic activity of hemin/G-quadruplex (DNAzyme). When human immunodeficiency virus (HIV) DNA biomarker was used as the model analyst, a naked-eye detection with high selectivity and high sensitivity down to 10-17 M in whole serum was achieved by observing red-to-blue color change. Single-base mismatch and two-base mismatch were detected at the low concentrations of 10-11 and 10-8 M, respectively. The naked-eye detection based on the enzyme-free plasmonic nanoplatform is expected to have potential applications ranging from quick detection and early diagnostics to point-of-care research.

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Citations

Aug 7, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaoling LiCuisong Zhou
Jan 29, 2021·Analytical and Bioanalytical Chemistry·Lin ZhuChaoyong Yang
Jul 14, 2021·Chemical Reviews·Min LiQiang Xia
Oct 28, 2019·Analytical Chemistry·Erin Marie McConnellYingfu Li

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